Pseudochrobactrum albidum sp. nov., a N,N-dimethylformamide-Degrading Bacterium Isolated from Activated Sludge of a Wastewater Treatment Plant
摘要
A N,N-dimethylformamide-degrading bacterium, strain HB0163ᵀ, was isolated from activated sludge of a wastewater treatment plant in Hangzhou, Zhejiang Province, China. Cells are Gram-stain-negative, aerobic, oxidase-positive, non-motile rods (0.4–0.6 × 1.0–1.5 μm). Growth occurred at 20–45 °C (optimum 35 °C), pH 5.0–9.5 (optimum 7.5–8.0), and with 0–6.0% (w/v) NaCl (optimum 2.0–3.0%). Chemotaxonomic analysis showed Ubiquinone-10 as the respiratory quinone, C19:0 cyclo ω8c, C18:1 ω7c and C16:0 as major fatty acids, and a polar lipid profile consisting of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, two unknown phosphoglycolipids, three unknown aminolipids, two unknown phospholipids, and one unknown lipid. The genomic DNA G + C content was 50.7%. Phylogenetic analysis of the 16S rRNA gene indicated its placement within the genus Pseudochrobactrum. Average nucleotide identity and digital DNA–DNA hybridization values between HB0163ᵀ and its closest relatives were 80.4–81.1% (< 95%) and 22.7–23.4% (< 70%), respectively, below the established species thresholds. Based on the polyphasic characterization, strain HB0163ᵀ represents a novel species of the genus Pseudochrobactrum, for which the name Pseudochrobactrum albidum sp. nov. is proposed. The type strain is HB0163ᵀ (= MCCC 1K09177ᵀ = JCM 37008ᵀ).