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<p>A cream-white bacterial colony designated SH1-B was isolated on Tryptone Soya Agar (TSA) plated with a brackish water sample collected from the Pichavaram mangrove forest. The strain SH1-B was Gram-stain negative, rod-shaped, motile, non-spore-forming aerobic bacterium. Catalase and oxidase activities were positive. Cells grew between 15 and 37&#xa0;°C and pH 5.0–9.0. The nucleotide sequence of 16&#xa0;S rRNA gene displayed high similarity with species of the genus <i>Pseudomonas</i>, including <i>P. khazarica</i> TBZ2<sup>T</sup> (98.5%), <i>P</i>. <i>yangonensis</i> MY50<sup>T</sup> (98.0%) and <i>P. oleovorans</i> subsp. <i>oleovorans</i> DSM1045<sup>T</sup> (98.0%). Genome sequence analysis of strain SH1-B showed 74.2–87.6% average nucleotide identity (ANI) with the type strains of the closest species. Additionally, digital DNA-DNA hybridization (dDDH) analysis revealed low relatedness, with a maximum value of 34.9% observed for its closest phylogenetic relative, <i>P. khazarica</i> TBZ2<sup>T</sup>. The G + C content of the genomic DNA was 62.2%. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and an unidentified phospholipid (PL1). The predominant cellular fatty acids of strain SH1-B included C<sub>18:1</sub><i>ω</i>7<i>c</i>, C<sub>16:0,</sub> C<sub>12:0,</sub> summed feature 3 (C<sub>16:1</sub><i>ω</i>6<i>c</i>/C<sub>16:1</sub><i>ω</i>7<i>c</i>). Considering the phenotypic differences and low genomic identity compared to the closest species, strain SH1-B was identified as a novel species within the genus <i>Pseudomonas</i>, for which the name <i>Pseudomonas pichavaramensis</i> sp. nov. is proposed. The type strain is SH1-B<sup>T</sup> (= MTCC 13546<sup>T</sup> = JCM 37389<sup>T</sup> = ICMP 25819<sup>T</sup>).</p>

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Pseudomonas Pichavaramensis sp. nov., Isolated from Brackish Water of Pichavaram Mangrove forest, Tamil Nadu

  • 期刊论文

  • 发表日期：

    2026年2月16日

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  • Sumit Mittal,
  • Hariom Kushwaha,
  • Nandini,
  • Stanzin Choksket,
  • Devachandana C. Prabhu,
  • Anil Kumar Pinnaka,
  • Suresh Korpole

摘要

A cream-white bacterial colony designated SH1-B was isolated on Tryptone Soya Agar (TSA) plated with a brackish water sample collected from the Pichavaram mangrove forest. The strain SH1-B was Gram-stain negative, rod-shaped, motile, non-spore-forming aerobic bacterium. Catalase and oxidase activities were positive. Cells grew between 15 and 37 °C and pH 5.0–9.0. The nucleotide sequence of 16 S rRNA gene displayed high similarity with species of the genus Pseudomonas, including P. khazarica TBZ2T (98.5%), P. yangonensis MY50T (98.0%) and P. oleovorans subsp. oleovorans DSM1045T (98.0%). Genome sequence analysis of strain SH1-B showed 74.2–87.6% average nucleotide identity (ANI) with the type strains of the closest species. Additionally, digital DNA-DNA hybridization (dDDH) analysis revealed low relatedness, with a maximum value of 34.9% observed for its closest phylogenetic relative, P. khazarica TBZ2T. The G + C content of the genomic DNA was 62.2%. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and an unidentified phospholipid (PL1). The predominant cellular fatty acids of strain SH1-B included C18:1ω7c, C16:0, C12:0, summed feature 3 (C16:1ω6c/C16:1ω7c). Considering the phenotypic differences and low genomic identity compared to the closest species, strain SH1-B was identified as a novel species within the genus Pseudomonas, for which the name Pseudomonas pichavaramensis sp. nov. is proposed. The type strain is SH1-BT (= MTCC 13546T = JCM 37389T = ICMP 25819T).