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<p>A Gram-stain-negative, obligately aerobic, beige and ovoid bacterium, designated strain SDUM112013<sup>T</sup>, was isolated from intertidal sediments of Xiaoshi Island, Weihai, China. Growth occurred at 20–37&#xa0;°C (optimal 28–35&#xa0;°C), at pH 6.0–9.0 (optimal pH 7.0), in the presence of 1–7% (w/v) NaCl (optimal 3–4%). Based on 16S rRNA gene sequence comparisons, strain SDUM112013<sup>T</sup> showed highest similarity to <i>Shimia sediminis</i> KCTC 62578<sup>T</sup> (98.12%), followed by <i>Shimia aestuarii</i> JCM 14332<sup>T</sup> (97.91%). Phylogenetic analysis confirmed its affiliation within the genus <i>Shimia</i>. The digital DNA-DNA hybridization (dDDH) values between strain SDUM112013<sup>T</sup> and <i>S. aestuarii</i> JCM 14332<sup>T</sup> (20.5%), <i>S. marina</i> JCM 13038<sup>T</sup> (19.70%) were significantly below the 70% species threshold. Consistently, average nucleotide identity (ANI) values between strain SDUM112013<sup>T</sup> and <i>S. aestuarii</i> JCM 14332<sup>T</sup> (75.82%), <i>S. marina</i> JCM 13038<sup>T</sup> (73.10%) also fell below the novel species boundary (95%). The genome of strain SDUM112013<sup>T</sup> (3,823,137&#xa0;bp; G + C content 59.8%) contained a complete ectoine biosynthesis (M00033) pathway, and represents the first characterized member of the genus <i>Shimia</i> possessing this genetic trait, potentially reflecting unique adaptive evolution. Chemotaxonomic analysis identified ubiquinone-10 as the sole respiratory quinone. Major fatty acids (&gt; 5.0%) included C<sub>16:0</sub>, C<sub>17:0</sub>, C<sub>18:1</sub> <i>ω</i>7c 11-methyl and summed feature 8 (C<sub>18:1</sub> <i>ω</i>7c and/or C<sub>18:1</sub> <i>ω</i>6c). Polar lipids were consisted of phosphatidylglycerol, phosphatidyldimethylethanolamine diphosphatidylglycerol and phos-phatidylethanolamine. Based on the evidence presented in this study, strain SDUM112013<sup>T</sup> represents a novel species of the genus <i>Shimia</i>, for which the name <i>Shimia fortis</i> sp. nov. is proposed. The type strain is SDUM112013<sup>T</sup> (= KCTC 8307<sup>T</sup> = MCCC 1H01481<sup>T</sup>).</p>

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Shimia fortis sp. nov., Isolated from Marine Sediment

  • 期刊论文

  • 发表日期：

    2026年1月19日

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  • Xiao-Yu Dong,
  • Pei-Ran Lin,
  • Xin-Yun Tan,
  • Jia-Qi Li,
  • Zong-Jun Du,
  • Meng-Qi Ye

摘要

A Gram-stain-negative, obligately aerobic, beige and ovoid bacterium, designated strain SDUM112013T, was isolated from intertidal sediments of Xiaoshi Island, Weihai, China. Growth occurred at 20–37 °C (optimal 28–35 °C), at pH 6.0–9.0 (optimal pH 7.0), in the presence of 1–7% (w/v) NaCl (optimal 3–4%). Based on 16S rRNA gene sequence comparisons, strain SDUM112013T showed highest similarity to Shimia sediminis KCTC 62578T (98.12%), followed by Shimia aestuarii JCM 14332T (97.91%). Phylogenetic analysis confirmed its affiliation within the genus Shimia. The digital DNA-DNA hybridization (dDDH) values between strain SDUM112013T and S. aestuarii JCM 14332T (20.5%), S. marina JCM 13038T (19.70%) were significantly below the 70% species threshold. Consistently, average nucleotide identity (ANI) values between strain SDUM112013T and S. aestuarii JCM 14332T (75.82%), S. marina JCM 13038T (73.10%) also fell below the novel species boundary (95%). The genome of strain SDUM112013T (3,823,137 bp; G + C content 59.8%) contained a complete ectoine biosynthesis (M00033) pathway, and represents the first characterized member of the genus Shimia possessing this genetic trait, potentially reflecting unique adaptive evolution. Chemotaxonomic analysis identified ubiquinone-10 as the sole respiratory quinone. Major fatty acids (> 5.0%) included C16:0, C17:0, C18:1 ω7c 11-methyl and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). Polar lipids were consisted of phosphatidylglycerol, phosphatidyldimethylethanolamine diphosphatidylglycerol and phos-phatidylethanolamine. Based on the evidence presented in this study, strain SDUM112013T represents a novel species of the genus Shimia, for which the name Shimia fortis sp. nov. is proposed. The type strain is SDUM112013T (= KCTC 8307T = MCCC 1H01481T).