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<p><i>Vibrio hyugaensis</i> is an opportunistic pathogen widely distributed in marine environments. Phages are considered an important method for controlling pathogenic bacteria. However, phages infecting <i>V. hyugaensis</i> have not yet been reported. In this study, a novel phage infecting <i>V. hyugaensis</i>, named vB_VhyS_SJ1, was isolated from nearshore surface seawater of Qingdao, China. Transmission electron microscopy showed that it had a siphoviral morphology. vB_VhyS_SJ1 exhibits a latent period of 10&#xa0;min and a burst size of 52 ± 2 PFU/infected bacterium. It is stable over a temperature range of 4&#xa0;°C to 65&#xa0;°C and remains stable within a pH range of 5 to 10. The inhibition ability of the vB_VhyS_SJ1 against the <i>V. hyugaensis</i> was tested in vitro using the vB_VhyS_SJ1 with four multiplicity of infection (MOI) values. All treated groups effectively inhibited the growth compared to the untreated culture of <i>V. hyugaensis</i> within 12&#xa0;h. The genome of vB_VhyS_SJ1 is double-stranded DNA, with a genome size of 121,361&#xa0;bp, containing 174 putative open reading frames (ORFs). Four auxiliary metabolic genes (AMGs) were predicted, and a total of 25 tRNA genes were identified. Phylogenetic and genomic network analysis demonstrated that vB_VhyS_SJ1 belongs to the <i>Pogseptimavirus</i> genus of the <i>Demerecviridae</i> family. Additionally, biogeographical distribution analysis found that vB_VhyS_SJ1 is primarily found in temperate, and tropical epipelagic zones with low abundance. Overall, this study provides a characterization of the first phage infecting the opportunistic pathogenic <i>V. hyugaensis.</i></p>

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Characterization and Genomic Analysis of the First Phage Infecting Vibrio hyugaensis, vB_VhyS_SJ1

  • 期刊论文

  • 发表日期：

    2025年12月22日

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  • Suqing Zhang,
  • Wei Wang,
  • Xin Chen,
  • Ziyue Wang,
  • Xiangyun Chen,
  • Zhaobin Zheng,
  • Ni Wang,
  • Yeong Yik Sung,
  • Wen Jye Mok,
  • Li Lian Wong,
  • Min Wang,
  • Hongbing Shao,
  • Yundan Liu,
  • Yantao Liang

摘要

Vibrio hyugaensis is an opportunistic pathogen widely distributed in marine environments. Phages are considered an important method for controlling pathogenic bacteria. However, phages infecting V. hyugaensis have not yet been reported. In this study, a novel phage infecting V. hyugaensis, named vB_VhyS_SJ1, was isolated from nearshore surface seawater of Qingdao, China. Transmission electron microscopy showed that it had a siphoviral morphology. vB_VhyS_SJ1 exhibits a latent period of 10 min and a burst size of 52 ± 2 PFU/infected bacterium. It is stable over a temperature range of 4 °C to 65 °C and remains stable within a pH range of 5 to 10. The inhibition ability of the vB_VhyS_SJ1 against the V. hyugaensis was tested in vitro using the vB_VhyS_SJ1 with four multiplicity of infection (MOI) values. All treated groups effectively inhibited the growth compared to the untreated culture of V. hyugaensis within 12 h. The genome of vB_VhyS_SJ1 is double-stranded DNA, with a genome size of 121,361 bp, containing 174 putative open reading frames (ORFs). Four auxiliary metabolic genes (AMGs) were predicted, and a total of 25 tRNA genes were identified. Phylogenetic and genomic network analysis demonstrated that vB_VhyS_SJ1 belongs to the Pogseptimavirus genus of the Demerecviridae family. Additionally, biogeographical distribution analysis found that vB_VhyS_SJ1 is primarily found in temperate, and tropical epipelagic zones with low abundance. Overall, this study provides a characterization of the first phage infecting the opportunistic pathogenic V. hyugaensis.