Soil salinization, a major cause of soil degradation, induces osmotic and oxidative stresses in plants and impairs growth and yield. PGPB possess unique functions, such as production of growth-promoting substances like auxin (IAA) and the ability to dissolve soil nutrients, which could help plants resist saline-alkali stress. This study investigated the protective effect of Bacillus amyloliquefaciens YB1701 on rice subjected to Na2CO3 stress (0–20 mM) through hydroponic co-culture of rice seedlings with bacterial suspensions. On the 7th day of cultivation, morphological characteristics and physiological indicators were measured. The results showed that under all stress concentrations, rice inoculated with strain YB1701 grew better than non-inoculated group, with increased shoot biomass. This linked to the improvement of rice leaves in antioxidant capacity with increased activities of SOD, POD, and CAT and reduced ROS levels. At 15 mM Na2CO3, the <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="284_2025_4560_Article_IEq1.gif" Format="GIF" Height="20" Rendition="HTML" Resolution="72" Type="Linedraw" Width="29" /> </InlineMediaObject> <EquationSource Format="TEX">${\text{O}}_{2}^{\cdot -}$</EquationSource> <EquationSource Format="MATHML"><math> <msubsup> <mtext>O</mtext> <mrow> <mn>2</mn> </mrow> <mrow> <mo>·</mo> <mo>-</mo> </mrow> </msubsup> </math></EquationSource> </InlineEquation> and H2O2 decreased by 32.06% and 15.66%, respectively, compared with the control group, which was one of the reasons for the reduced cell membrane damage. Strain YB1701 reduced the Na⁺/K⁺ ratio in rice leaves and roots, alleviating osmotic stress caused by Na2CO3: decreasing by 32.24% and 39.49% at 10 mM Na₂CO3. Additionally, strain YB1701 increased photosynthetic pigment contents and chlorophyll fluorescence and protected photosynthesis. Therefore, strain YB1701 improves rice growth by alleviating osmotic/oxidative stress and promoting photosynthesis, providing a new biological solution for the stable production of rice in saline-alkali soil.

Bacillus amyloliquefaciens YB1701 Enhances Rice Tolerance to Saline-Alkali Stress Via ROS Scavenging and Ion Homeostasis

期刊论文
发表日期：

2025年10月31日

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Enjing Yi,
Jing Zhang,
Yuping Jiang,
Leibei Yang,
Mengyu Sun,
Lanlan Wang,
Xuemei Li,
Yueying Li,
Yuzhu Dong,
Lianju Ma

摘要

Soil salinization, a major cause of soil degradation, induces osmotic and oxidative stresses in plants and impairs growth and yield. PGPB possess unique functions, such as production of growth-promoting substances like auxin (IAA) and the ability to dissolve soil nutrients, which could help plants resist saline-alkali stress. This study investigated the protective effect of Bacillus amyloliquefaciens YB1701 on rice subjected to Na₂CO₃ stress (0–20 mM) through hydroponic co-culture of rice seedlings with bacterial suspensions. On the 7th day of cultivation, morphological characteristics and physiological indicators were measured. The results showed that under all stress concentrations, rice inoculated with strain YB1701 grew better than non-inoculated group, with increased shoot biomass. This linked to the improvement of rice leaves in antioxidant capacity with increased activities of SOD, POD, and CAT and reduced ROS levels. At 15 mM Na₂CO₃, the ${\text{O}}_{2}^{\cdot -}$ $O_{2}^{\cdot -}$ and H₂O₂ decreased by 32.06% and 15.66%, respectively, compared with the control group, which was one of the reasons for the reduced cell membrane damage. Strain YB1701 reduced the Na⁺/K⁺ ratio in rice leaves and roots, alleviating osmotic stress caused by Na₂CO₃: decreasing by 32.24% and 39.49% at 10 mM Na₂CO₃. Additionally, strain YB1701 increased photosynthetic pigment contents and chlorophyll fluorescence and protected photosynthesis. Therefore, strain YB1701 improves rice growth by alleviating osmotic/oxidative stress and promoting photosynthesis, providing a new biological solution for the stable production of rice in saline-alkali soil.