Scholarlink

<p>The transmission of life-threatening bacteria with plasmid-mediated antibiotic resistance poses a significant challenge to public health. This study aimed to determine the presence of plasmid-mediated antibiotic resistance genes in Enterobacterales isolates obtained from dried fishery products. Eighty-one dried fishery products were purchased from Vietnamese markets. Enterobacterales were isolated using a CHROMagar <i>Escherichia coli</i> coliform agar containing cefotaxime or meropenem. The isolated strains were assessed for their susceptibility to 14 antibiotics using a disc diffusion assay. Extended-spectrum β-lactamase (ESBL) sub-group typing was performed based on multiplex PCR of isolated ESBL-producing strains. In addition, <i>Enterobacter cloacae</i> AD2-1, which showed multiple drug resistance, was subject to whole-genome sequence analysis. CTX-resistant bacteria were isolated from 22% and MEM-resistant bacteria from 27% of the Vietnamese samples. CTX-resistant bacteria were isolated from 17% and MEM-resistant bacteria from 4% of Japanese samples. Bacterial identification indicated that 98 strains were isolated, of which 29 strains of <i>E. coli</i>, 28 of <i>Enterobacter cloacae</i> complex, 19 of <i>Staphylococcus</i> spp., and 9 of <i>Klebsiella pneumoniae</i> were predominant in Vietnamese samples. Japanese samples were predominantly contaminated with <i>E. cloacae</i> complex. Multiplex PCR and sequencing was used to determine the presence of ESBL-related genes <i>bla</i><sub>CTX-M-15</sub> and <i>bla</i><sub>CTX-M-55</sub> in <i>E. cloacae</i> and <i>K. pneumoniae</i> isolates. <i>E. cloacae</i> AD2-1 isolated from the Vietnamese dried fish was resistant to 14 antibiotics, and approximately 300 kbp of the IncHI2 plasmid harboured multiple antibiotic resistance genes and formed an antibiotic resistance gene region. This <i>E. cloacae</i> is considered a risk for the spread of antibiotic resistance across countries.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

ESBL-Producing Enterobacter cloacae Complex and Klebsiella pneumoniae Harbouring blaCTX-M-15 and blaCTX-M-55 Potentially Risk the Worldwide Spread of ESBL-Producing Bacteria Through Contaminated Dried Fishery Products

  • 期刊论文

  • 发表日期：

    2025年10月31日

查看全文
  • Tatsuya Nakayama,
  • Yuki Kasumi,
  • Machika Saito,
  • Natsuki Ohata,
  • Takahiro Yamaguchi,
  • Michio Jinnai,
  • Yuko Kumeda,
  • Atsushi Hase

摘要

The transmission of life-threatening bacteria with plasmid-mediated antibiotic resistance poses a significant challenge to public health. This study aimed to determine the presence of plasmid-mediated antibiotic resistance genes in Enterobacterales isolates obtained from dried fishery products. Eighty-one dried fishery products were purchased from Vietnamese markets. Enterobacterales were isolated using a CHROMagar Escherichia coli coliform agar containing cefotaxime or meropenem. The isolated strains were assessed for their susceptibility to 14 antibiotics using a disc diffusion assay. Extended-spectrum β-lactamase (ESBL) sub-group typing was performed based on multiplex PCR of isolated ESBL-producing strains. In addition, Enterobacter cloacae AD2-1, which showed multiple drug resistance, was subject to whole-genome sequence analysis. CTX-resistant bacteria were isolated from 22% and MEM-resistant bacteria from 27% of the Vietnamese samples. CTX-resistant bacteria were isolated from 17% and MEM-resistant bacteria from 4% of Japanese samples. Bacterial identification indicated that 98 strains were isolated, of which 29 strains of E. coli, 28 of Enterobacter cloacae complex, 19 of Staphylococcus spp., and 9 of Klebsiella pneumoniae were predominant in Vietnamese samples. Japanese samples were predominantly contaminated with E. cloacae complex. Multiplex PCR and sequencing was used to determine the presence of ESBL-related genes blaCTX-M-15 and blaCTX-M-55 in E. cloacae and K. pneumoniae isolates. E. cloacae AD2-1 isolated from the Vietnamese dried fish was resistant to 14 antibiotics, and approximately 300 kbp of the IncHI2 plasmid harboured multiple antibiotic resistance genes and formed an antibiotic resistance gene region. This E. cloacae is considered a risk for the spread of antibiotic resistance across countries.