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<p>Diseases caused by Begomovirus are a severe obstacle for dicotyledonous plants worldwide. In Bihar, a northern Indian state, we have been seeing indicators of stunting and chlorosis in lentil plants, with an incidence of 2.90%. Based on the series of tests between 2021 and 2024, the suspected organism was determined to be Begomovirus<i>.</i> After restriction digestion, <i>HindIII</i> and <i>BglI</i>, the RCA of three DNA samples revealed a linearized DNA of about ~ 2.7&#xa0;kb. With a genomic organization consisting of two ORFs in virion-sense and five ORFs in complementary sense separated by IR region, the associated virus was discovered to have 2746 nucleotide for DNA-A. When compared to the isolates of Mungbean yellow mosaic India virus (MYMIV), the genome sequences of DNA-A and DNA-B exhibited the highest nucleotide identity (98%). A single recombination event has been detected in DNA-A, with the major and minor parent classified as MYMIV isolates from Bangladesh and Paskistan, respectively. Two overlapping primers were designed using nucleotide sequence of MYMIV-Lentil isolate in order to amplify the virus’s genome. DNA-A and DNA-B showed PCR-mediated amplification rates of 70.8 and 69.6%, respectively, when both primer sets were used. In order to ensure linked virus, whitefly-mediated transmission was tried in a controlled environment. In five examined lentil types, a meagre transmission rate (6.1–7.8%) was observed. The present evidence that a virus infects lentil crop rather than other microbe that cause stunting offers valuable insight for developing effective management measures.</p>

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Molecular Evidence of Chlorotic Stunt Disease Induced by Mungbean yellow mosaic India virus (MYMIV) in Lentil (Lens esculentus)

  • 期刊论文

  • 发表日期：

    2025年10月24日

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  • Mohammad Ansar,
  • Vikash Kumar,
  • Tushar Ranjan,
  • Mohammad Akram,
  • Jitendra Nath Srivastava,
  • Rashid Mumtaz Khan,
  • Md Arshad Anwer,
  • Ravi Ranjan Kumar,
  • Mohammad Feza Ahmad,
  • Arun Prasad Bhagat

摘要

Diseases caused by Begomovirus are a severe obstacle for dicotyledonous plants worldwide. In Bihar, a northern Indian state, we have been seeing indicators of stunting and chlorosis in lentil plants, with an incidence of 2.90%. Based on the series of tests between 2021 and 2024, the suspected organism was determined to be Begomovirus. After restriction digestion, HindIII and BglI, the RCA of three DNA samples revealed a linearized DNA of about ~ 2.7 kb. With a genomic organization consisting of two ORFs in virion-sense and five ORFs in complementary sense separated by IR region, the associated virus was discovered to have 2746 nucleotide for DNA-A. When compared to the isolates of Mungbean yellow mosaic India virus (MYMIV), the genome sequences of DNA-A and DNA-B exhibited the highest nucleotide identity (98%). A single recombination event has been detected in DNA-A, with the major and minor parent classified as MYMIV isolates from Bangladesh and Paskistan, respectively. Two overlapping primers were designed using nucleotide sequence of MYMIV-Lentil isolate in order to amplify the virus’s genome. DNA-A and DNA-B showed PCR-mediated amplification rates of 70.8 and 69.6%, respectively, when both primer sets were used. In order to ensure linked virus, whitefly-mediated transmission was tried in a controlled environment. In five examined lentil types, a meagre transmission rate (6.1–7.8%) was observed. The present evidence that a virus infects lentil crop rather than other microbe that cause stunting offers valuable insight for developing effective management measures.