Scholarlink

<p>Two Gram-stain-negative, catalase- and oxidase-positive, non-motile strains, S98ᵀ and JB4-11ᵀ, were isolated from marine red algae. Strain S98ᵀ was a strictly aerobic, ovoid to short rod-shaped bacterium that grew at 15–30 °C, pH 7.0–9.0, and 1.0–4.0% (w/v) NaCl. In contrast, strain JB4-11ᵀ was a facultatively anaerobic, rod-shaped bacterium that grew at 20–30 °C, pH 7.0–10.0, and 1.0–7.0% (w/v) NaCl. Both strains contained phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, and an unidentified phospholipid as major polar lipids, with JB4-11ᵀ additionally possessing diphosphatidylglycerol. The predominant fatty acids were summed feature 8 (C<sub>18:1</sub> <i>ω</i>7<i>c</i> and/or C<sub>18:1</sub> <i>ω</i>6<i>c</i>) and C<sub>16:0</sub>, and both strains contained ubiquinone-10 as the sole isoprenoid quinone. The genomic DNA G + C content was 60.0% for S98ᵀ and 60.6% for JB4-11ᵀ. Strains S98ᵀ and JB4-11ᵀ shared 97.3% 16S rRNA gene sequence similarity and formed distinct lineages from other <i>Roseobacter</i> and <i>Sulfitobacter</i> species, with ≤ 98.1% similarity, in 16S rRNA gene-based phylogenetic trees. Genome-based phylogenetic analyses revealed that strains S98ᵀ and JB4-11ᵀ clustered with <i>Roseobacter cerasinus</i> AI77ᵀ and <i>Sulfitobacter aestuariivivens</i> TSTF-M16ᵀ within the genera <i>Roseobacter</i> and <i>Sulfitobacter</i>, respectively. Average nucleotide identity and digital DNA-DNA hybridization values with other <i>Roseobacter</i> and <i>Sulfitobacter</i> species were below 75.9% and 19.7%, respectively. Based on phenotypic, chemotaxonomic, and genomic characteristics, strains S98ᵀ and JB4-11ᵀ represent novel species of <i>Roseobacter</i> and <i>Sulfitobacter</i>, for which the names <i>Roseobacter algicola</i> sp. nov. and <i>Sulfitobacter rhodophyticola</i> sp. nov. are proposed. The type strains are S98ᵀ (= KACC 23419ᵀ = JCM 36648ᵀ) and JB4-11ᵀ (= KACC 23867ᵀ = JCM 36646ᵀ), respectively.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Roseobacter algicola sp. nov. and Sulfitobacter rhodophyticola sp. nov., Isolated from Marine Red Algae

  • 期刊论文

  • 发表日期：

    2025年9月16日

查看全文
  • Seo Hui Choi,
  • Woonhee Baek,
  • Jeong Min Kim,
  • Byeong Jun Choi,
  • Hülya Bayburt,
  • Jae Kyeong Lee,
  • Sung Chul Lee,
  • Che Ok Jeon

摘要

Two Gram-stain-negative, catalase- and oxidase-positive, non-motile strains, S98ᵀ and JB4-11ᵀ, were isolated from marine red algae. Strain S98ᵀ was a strictly aerobic, ovoid to short rod-shaped bacterium that grew at 15–30 °C, pH 7.0–9.0, and 1.0–4.0% (w/v) NaCl. In contrast, strain JB4-11ᵀ was a facultatively anaerobic, rod-shaped bacterium that grew at 20–30 °C, pH 7.0–10.0, and 1.0–7.0% (w/v) NaCl. Both strains contained phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, and an unidentified phospholipid as major polar lipids, with JB4-11ᵀ additionally possessing diphosphatidylglycerol. The predominant fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C16:0, and both strains contained ubiquinone-10 as the sole isoprenoid quinone. The genomic DNA G + C content was 60.0% for S98ᵀ and 60.6% for JB4-11ᵀ. Strains S98ᵀ and JB4-11ᵀ shared 97.3% 16S rRNA gene sequence similarity and formed distinct lineages from other Roseobacter and Sulfitobacter species, with ≤ 98.1% similarity, in 16S rRNA gene-based phylogenetic trees. Genome-based phylogenetic analyses revealed that strains S98ᵀ and JB4-11ᵀ clustered with Roseobacter cerasinus AI77ᵀ and Sulfitobacter aestuariivivens TSTF-M16ᵀ within the genera Roseobacter and Sulfitobacter, respectively. Average nucleotide identity and digital DNA-DNA hybridization values with other Roseobacter and Sulfitobacter species were below 75.9% and 19.7%, respectively. Based on phenotypic, chemotaxonomic, and genomic characteristics, strains S98ᵀ and JB4-11ᵀ represent novel species of Roseobacter and Sulfitobacter, for which the names Roseobacter algicola sp. nov. and Sulfitobacter rhodophyticola sp. nov. are proposed. The type strains are S98ᵀ (= KACC 23419ᵀ = JCM 36648ᵀ) and JB4-11ᵀ (= KACC 23867ᵀ = JCM 36646ᵀ), respectively.