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<p>The study tested the hypothesis that cultivation conditions and additional exposure to light radiation influence the magnitude of the antibacterial effect of non-polar metabolites extracted from the cells of the microalgae <i>Chlorella sorokiniana</i> and the cyanobacteria <i>Anabaena sphaerica</i>. It was found that non-polar extracts of the microalgae <i>Chlorella sorokiniana</i> and the cyanobacteria <i>Anabaena sphaerica</i> extracted with petroleum ether have an inhibitory effect on the growth of Gram-positive bacteria of the genus <i>Bacillus</i>. The most promising antibacterial agents are extracts of microalgae and cyanobacteria obtained from cell biomass grown at the level of photosynthetically active radiation 100 ± 10&#xa0;µmol photons/(m<sup>2</sup>s) and temperature – 36 ± 0.1&#xa0;°C. At the same time, the non-polar extract of the microalgae <i>Chlorella sorokiniana</i> has an inhibitory effect only when the system is additionally exposed to light (100 ± 10&#xa0;µmol photons/(m<sup>2</sup>·s)) (the minimum inhibitory concentration of the extract is ≈&#xa0;125&#xa0;µg/ml), whereas the non-polar extract of the cyanobacteria <i>Anabaena sphaerica</i> shows antimicrobial activity both with and without additional light radiation (the minimum inhibitory concentration of the extract is 72&#xa0;µg/ml and 830&#xa0;µg/ml, respectively). For these extracts, IC<sub>50</sub> concentration values for the HEK293 cell line and SI selectivity indices for <i>Chlorella sorokiniana</i> (IC<sub>50</sub> = 260&#xa0;µg/ml, SI (‘light’) = 2.08) and <i>Anabaena sphaerica</i> (IC<sub>50</sub> = 220&#xa0;µg/ml, SI = 3.06/0.27 (‘light’/‘dark’)) were determined.</p>

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Investigating the Impact of Cultivation Regimes on the Antibacterial Properties of Non-Polar Extracts of Chlorella sorokiniana and Anabaena sphaerica

  • 期刊论文

  • 发表日期：

    2025年9月12日

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  • Mikhail S. Temnov,
  • Yana V. Ustinskaya,
  • Kirill I. Meronyuk,
  • Alexandra K. Bryankina,
  • Dmitry S. Dvoretsky

摘要

The study tested the hypothesis that cultivation conditions and additional exposure to light radiation influence the magnitude of the antibacterial effect of non-polar metabolites extracted from the cells of the microalgae Chlorella sorokiniana and the cyanobacteria Anabaena sphaerica. It was found that non-polar extracts of the microalgae Chlorella sorokiniana and the cyanobacteria Anabaena sphaerica extracted with petroleum ether have an inhibitory effect on the growth of Gram-positive bacteria of the genus Bacillus. The most promising antibacterial agents are extracts of microalgae and cyanobacteria obtained from cell biomass grown at the level of photosynthetically active radiation 100 ± 10 µmol photons/(m2s) and temperature – 36 ± 0.1 °C. At the same time, the non-polar extract of the microalgae Chlorella sorokiniana has an inhibitory effect only when the system is additionally exposed to light (100 ± 10 µmol photons/(m2·s)) (the minimum inhibitory concentration of the extract is ≈ 125 µg/ml), whereas the non-polar extract of the cyanobacteria Anabaena sphaerica shows antimicrobial activity both with and without additional light radiation (the minimum inhibitory concentration of the extract is 72 µg/ml and 830 µg/ml, respectively). For these extracts, IC50 concentration values for the HEK293 cell line and SI selectivity indices for Chlorella sorokiniana (IC50 = 260 µg/ml, SI (‘light’) = 2.08) and Anabaena sphaerica (IC50 = 220 µg/ml, SI = 3.06/0.27 (‘light’/‘dark’)) were determined.