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<p>A Gram-stain-positive, aerobic and drought- and salt-resistant bacterium, designated as N15<sup>T</sup>, was isolated from a saline-alkali soil in Songyuan city, Jilin Province, China. The strain grew in the presence of 0–12% (w/v) NaCl, at pH 6.0–8.0 and 15–42&#xa0;°C; optimum growth was observed with 6.0% (w/v) NaCl, at pH 7.0 and 30&#xa0;°C. Strain N15<sup>T</sup> tolerated up to 45% PEG6000 and 12% NaCl, and could efficiently degrade aniline blue. Phylogenetic analysis based on 16S rRNA genes placed strain N15<sup>T</sup> within genus&#xa0;<i>Lentibacillus</i>, forming a subclade with <i>Lentibacillus daqui</i>&#xa0;ZS110521<sup>T</sup> (97.5% similarity) and&#xa0;<i>L. garicola</i>&#xa0;SL-MJ1<sup>T</sup> (95.9% similarity). The major cellular fatty acids (≥ 5%) were anteiso-C<sub>15:0</sub>, ananteiso-C<sub>17:0</sub> and iso-C<sub>17:0</sub>. The respiratory quinone was MK-7 and peptidoglycan contained <i>meso</i>-DAP. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The genome size of strain N15<sup>T</sup> was 4.5&#xa0;Mb, and the DNA G + C content was 40.1%. The nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain N15<sup>T</sup> and <i>L. daqui</i> ZS110521<sup>T</sup> were 77.8% and 22.6%, respectively, which were significantly below the recommended thresholds (ANI &lt; 95–96% and dDDH &lt; 70%) for species delineation. Based on the phenotypic, phylogenetic, chemotaxonomic, and genomic results from this study, strain N15<sup>T</sup> represents a novel species in genus <i>Lentibacillus</i>, for which the name <i>Lentibacillus songyuanensis</i> sp. nov. is proposed, the type strain is N15<sup>T</sup> (= MCCC 1K09143<sup>T</sup> = KCTC 43718<sup>T</sup>).</p>

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Lentibacillus songyuanensis sp. nov., A Drought- and Salt- Tolerant Bacterium with Ability to Degrade Aniline Blue Isolated from Saline-Alkali Soil

  • 期刊论文

  • 发表日期：

    2025年8月25日

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  • Enyi Wang,
  • Leyao Chen,
  • Leyun Dong,
  • Jian He,
  • Zongzhuan Shen,
  • Jiandong Jiang,
  • Qirong Shen

摘要

A Gram-stain-positive, aerobic and drought- and salt-resistant bacterium, designated as N15T, was isolated from a saline-alkali soil in Songyuan city, Jilin Province, China. The strain grew in the presence of 0–12% (w/v) NaCl, at pH 6.0–8.0 and 15–42 °C; optimum growth was observed with 6.0% (w/v) NaCl, at pH 7.0 and 30 °C. Strain N15T tolerated up to 45% PEG6000 and 12% NaCl, and could efficiently degrade aniline blue. Phylogenetic analysis based on 16S rRNA genes placed strain N15T within genus Lentibacillus, forming a subclade with Lentibacillus daqui ZS110521T (97.5% similarity) and L. garicola SL-MJ1T (95.9% similarity). The major cellular fatty acids (≥ 5%) were anteiso-C15:0, ananteiso-C17:0 and iso-C17:0. The respiratory quinone was MK-7 and peptidoglycan contained meso-DAP. The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The genome size of strain N15T was 4.5 Mb, and the DNA G + C content was 40.1%. The nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain N15T and L. daqui ZS110521T were 77.8% and 22.6%, respectively, which were significantly below the recommended thresholds (ANI < 95–96% and dDDH < 70%) for species delineation. Based on the phenotypic, phylogenetic, chemotaxonomic, and genomic results from this study, strain N15T represents a novel species in genus Lentibacillus, for which the name Lentibacillus songyuanensis sp. nov. is proposed, the type strain is N15T (= MCCC 1K09143T = KCTC 43718T).