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<p>Strain CNT-189<sup>Τ</sup>, previously isolated from marine sediment and identified as an androsamide producer, was taxonomically characterized in this study. Strain CNT-189<sup>Τ</sup> is a Gram-stain-positive, non-motile, pale yellow-pigmented, aerobic actinomycete. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNT-189<sup>Τ</sup> belonged to the genus <i>Nocardiopsis</i> and was most closely related to <i>Nocardiopsis potens</i> DSM 45234<sup>Τ</sup> (99.6% similarity). Whole-genome sequencing revealed a DNA G + C content of 74.1% and genome size of 7.12 Mbp. Average nucleotide identity (92.8–95.1%) and digital DNA-DNA hybridization values (45.9–55.4%) between strain CNT-189<sup>Τ</sup> and related species were below the species delineation thresholds, supporting its classification as a novel species. The major respiratory quinone was menaquinone-10 (MK-10) and the predominant cellular fatty acids were C<sub>18:1</sub> <i>ω</i>9<i>c</i> (25.0%), C<sub>16:0</sub> (13.5%), and anteiso-C<sub>15:0</sub> (12.7%). Major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylinositol mannoside, and monomannosyl diglyceride. Whole-cell hydrolysates contained meso-diaminopimelic acid, glycine, and no diagnostic sugars. Based on phylogenetic and phenotypic evidence, strain CNT-189<sup>T</sup> is considered to represent a novel species of the genus <i>Nocardiopsis</i>, for which the name <i>Nocardiopsis oceanisediminis</i> sp. nov. is proposed. The type strain is CNT-189<sup>Τ</sup> (= KACC 22136<sup>Τ</sup> = NBRC 114760<sup>Τ</sup>).</p>

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Nocardiopsis oceanisediminis sp. nov., a Marine-Derived Actinobacterium Isolated from Sediment

  • 期刊论文

  • 发表日期：

    2025年8月14日

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  • Mirae Kim,
  • Yeonjung Lim,
  • Jayho Lee,
  • Sang-Jip Nam,
  • Dong-Chan Oh,
  • Ilnam Kang,
  • Jang-Cheon Cho

摘要

Strain CNT-189Τ, previously isolated from marine sediment and identified as an androsamide producer, was taxonomically characterized in this study. Strain CNT-189Τ is a Gram-stain-positive, non-motile, pale yellow-pigmented, aerobic actinomycete. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CNT-189Τ belonged to the genus Nocardiopsis and was most closely related to Nocardiopsis potens DSM 45234Τ (99.6% similarity). Whole-genome sequencing revealed a DNA G + C content of 74.1% and genome size of 7.12 Mbp. Average nucleotide identity (92.8–95.1%) and digital DNA-DNA hybridization values (45.9–55.4%) between strain CNT-189Τ and related species were below the species delineation thresholds, supporting its classification as a novel species. The major respiratory quinone was menaquinone-10 (MK-10) and the predominant cellular fatty acids were C18:1 ω9c (25.0%), C16:0 (13.5%), and anteiso-C15:0 (12.7%). Major polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylinositol mannoside, and monomannosyl diglyceride. Whole-cell hydrolysates contained meso-diaminopimelic acid, glycine, and no diagnostic sugars. Based on phylogenetic and phenotypic evidence, strain CNT-189T is considered to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis oceanisediminis sp. nov. is proposed. The type strain is CNT-189Τ (= KACC 22136Τ = NBRC 114760Τ).