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<p>Selenium (Se) is a crucial trace element for human and animal health, contributing significantly in various physiological processes. However, its uneven global distribution leads to widespread deficiencies. Nano-selenium (selenium nanoparticles, SeNPs), with low toxicity and high bioavailability, emerges as a promising alternative, and microbial synthesis is the preferred method for its production. In this study, we collected samples from the selenium-rich region of Enshi, Hubei Province, China, and used sodium selenite (Na<sub>₂</sub>SeO<sub>₃</sub>) gradient screening to isolate microorganisms capable of tolerating high selenium concentrations and efficiently producing SeNPs. We identified nine selenium-tolerant strains that withstand over 0.115&#xa0;M of sodium selenite. Among these, strain HZ-9-3-3, identified as <i>Alcaligenes</i> sp<i>.</i>, tolerated up to 0.231&#xa0;M and effectively converted selenite to elemental selenium. Optimization using single-factor experiments and central composite design (CCD) established optimal conditions: inoculum size of 12.7% (±0.1%), pH 7, temperature 28&#xa0;°C, and sodium selenite concentration of 0.0289&#xa0;M, achieving a conversion rate of 69.7% (±0.1%). The produced SeNPs exhibited moderate antioxidant activity, with IC<sub>50</sub> values of 1.5&#xa0;mg/mL for DPPH radicals and 1.7&#xa0;mg/mL for hydroxyl radicals, and a reducing power equivalent to 0.842&#xa0;mg/mL of vitamin C. These findings highlight the potential of strain HZ-9-3-3 for efficient bioconversion of selenium and production of SeNPs with significant antioxidant properties.</p>

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Efficient Microbial Production of Selenium Nanoparticles by Selenium-Tolerant Alcaligenes sp. HZ-9-3-3

  • 期刊论文

  • 发表日期：

    2025年7月18日

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  • Omer Hassan Ali Hassan,
  • Ya-Ru Wei,
  • Zai-Ping Feng,
  • Jia-Mei Pei,
  • Jia-Nan Xu,
  • Wen-Tao Du,
  • Qiang-qiang Gou,
  • Wen-Di Wang,
  • Xue Zhao,
  • Qin-Cui Shi,
  • Xin-Guo Zhang

摘要

Selenium (Se) is a crucial trace element for human and animal health, contributing significantly in various physiological processes. However, its uneven global distribution leads to widespread deficiencies. Nano-selenium (selenium nanoparticles, SeNPs), with low toxicity and high bioavailability, emerges as a promising alternative, and microbial synthesis is the preferred method for its production. In this study, we collected samples from the selenium-rich region of Enshi, Hubei Province, China, and used sodium selenite (Na₂SeO₃) gradient screening to isolate microorganisms capable of tolerating high selenium concentrations and efficiently producing SeNPs. We identified nine selenium-tolerant strains that withstand over 0.115 M of sodium selenite. Among these, strain HZ-9-3-3, identified as Alcaligenes sp., tolerated up to 0.231 M and effectively converted selenite to elemental selenium. Optimization using single-factor experiments and central composite design (CCD) established optimal conditions: inoculum size of 12.7% (±0.1%), pH 7, temperature 28 °C, and sodium selenite concentration of 0.0289 M, achieving a conversion rate of 69.7% (±0.1%). The produced SeNPs exhibited moderate antioxidant activity, with IC50 values of 1.5 mg/mL for DPPH radicals and 1.7 mg/mL for hydroxyl radicals, and a reducing power equivalent to 0.842 mg/mL of vitamin C. These findings highlight the potential of strain HZ-9-3-3 for efficient bioconversion of selenium and production of SeNPs with significant antioxidant properties.