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<p><i>Streptococcus suis</i> is a zoonotic pathogen contributing to human and pig infections. Effective techniques to distinguish pathogenic from non-pathogenic pathotypes of <i>S. suis</i> is required for public health surveillance. Herein, we developed and evaluated a PCR approach detecting the human-associated clade (HAC)-G19 marker gene to identify the pathogenic pathotype of <i>S. suis</i>. The study was conducted on 599 human strains, 82 diseased-pig strains, and 299 clinically healthy pig strains. PCR analysis revealed that 98.8% and 75.6% were HAC-G19 positive in human and diseased-pig strains, whereas 67.6% of healthy pig strains were negative for the HAC-G19 gene. Statistical analysis revealed that the HAC-G19 gene was more significantly present in diseased-associated isolates than in healthy pig isolates (<i>p</i>-value &lt; 0.001), being with OR of 50.4 (95% CI = 32.1–79.5). Diagnostic accuracy was 87.4% (95% CI = 85.1–89.49%) with sensitivity and specificity were 96.1% (95% CI = 94.3–97.4%) and 67.6% (95% CI = 61.9–72.8%), respectively. This demonstrated that PCR of HAC-G19 has a potential to predict pathogenic pathotype for <i>S. suis</i>.</p>

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Presence of Human-Associated Clade Marker Gene HAC-G19 in Streptococcus suis Isolated From Humans and Swine

  • 期刊论文

  • 发表日期：

    2025年7月21日

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  • Rujirat Hatrongjit,
  • Kulsatri Sittichottumrong,
  • Nattakan Meekhanon,
  • Ratchadaporn Ungcharoen,
  • Zou Geng,
  • Parichart Boueroy,
  • Peechanika Chopjitt,
  • Marcelo Gottschalk,
  • Jinquan Li,
  • Anusak Kerdsin

摘要

Streptococcus suis is a zoonotic pathogen contributing to human and pig infections. Effective techniques to distinguish pathogenic from non-pathogenic pathotypes of S. suis is required for public health surveillance. Herein, we developed and evaluated a PCR approach detecting the human-associated clade (HAC)-G19 marker gene to identify the pathogenic pathotype of S. suis. The study was conducted on 599 human strains, 82 diseased-pig strains, and 299 clinically healthy pig strains. PCR analysis revealed that 98.8% and 75.6% were HAC-G19 positive in human and diseased-pig strains, whereas 67.6% of healthy pig strains were negative for the HAC-G19 gene. Statistical analysis revealed that the HAC-G19 gene was more significantly present in diseased-associated isolates than in healthy pig isolates (p-value < 0.001), being with OR of 50.4 (95% CI = 32.1–79.5). Diagnostic accuracy was 87.4% (95% CI = 85.1–89.49%) with sensitivity and specificity were 96.1% (95% CI = 94.3–97.4%) and 67.6% (95% CI = 61.9–72.8%), respectively. This demonstrated that PCR of HAC-G19 has a potential to predict pathogenic pathotype for S. suis.