Scholarlink

<p>Sesame phyllody disease poses a serious threat to sesame cultivation, often leading to complete crop failure due to the absence of resistant varieties and lack of effective chemical treatments. To explore sustainable alternatives, this study investigated the foliar microbiome by comparing microbial communities in healthy and phyllody-infected sesame plants from severely affected fields. Through combined culture-based techniques and DNA sequencing, we identified 3108 bacterial OTUs spanning several key genera, including <i>Pantoea</i>, <i>Pseudomonas</i>, <i>Allorhizobium</i>, and <i>Xanthomonas</i>. Phytoplasma, the known causal agent, was detected exclusively in symptomatic plants. Co-occurrence network analysis revealed distinct microbial associations: healthy plants showed balanced interactions with <i>Pantoea</i>, <i>Pseudomonas</i>, and <i>Sphingomonas</i> as central hubs, whereas infected plants exhibited a denser, more complex network, indicating phytoplasma-driven shifts in microbial structure. From culturable isolates, 72 bacterial strains were recovered, with <i>Pantoea</i> being predominant in both plant types. Functional predictions highlighted microbial association with nutrient metabolism and biosynthesis of alpha-linolenic acid, a naturally abundant compound in sesame. This first study of the sesame foliar microbiome provides valuable insights into plant-microbiome-phytoplasma interactions and lays a foundation for developing microbiome-based, eco-friendly strategies to manage sesame phyllody disease in the future.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Microbiome Assembly of Phyllody-Infected Sesame Leaves

  • 期刊论文

  • 发表日期：

    2025年7月21日

查看全文
  • Sakthivel Krishnan,
  • Charishma Krishnappa,
  • Neelam Sheoran,
  • Bhaskar Reddy,
  • Aundy Kumar

摘要

Sesame phyllody disease poses a serious threat to sesame cultivation, often leading to complete crop failure due to the absence of resistant varieties and lack of effective chemical treatments. To explore sustainable alternatives, this study investigated the foliar microbiome by comparing microbial communities in healthy and phyllody-infected sesame plants from severely affected fields. Through combined culture-based techniques and DNA sequencing, we identified 3108 bacterial OTUs spanning several key genera, including Pantoea, Pseudomonas, Allorhizobium, and Xanthomonas. Phytoplasma, the known causal agent, was detected exclusively in symptomatic plants. Co-occurrence network analysis revealed distinct microbial associations: healthy plants showed balanced interactions with Pantoea, Pseudomonas, and Sphingomonas as central hubs, whereas infected plants exhibited a denser, more complex network, indicating phytoplasma-driven shifts in microbial structure. From culturable isolates, 72 bacterial strains were recovered, with Pantoea being predominant in both plant types. Functional predictions highlighted microbial association with nutrient metabolism and biosynthesis of alpha-linolenic acid, a naturally abundant compound in sesame. This first study of the sesame foliar microbiome provides valuable insights into plant-microbiome-phytoplasma interactions and lays a foundation for developing microbiome-based, eco-friendly strategies to manage sesame phyllody disease in the future.