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<p>A Gram-stain-positive, aerobic, rod-shaped, and non-motile bacterium, designated strain R7<sup>T</sup>, was isolated from pig faeces. Strain R7<sup>T</sup> grew at 15–37&#xa0;°C (optimum 30&#xa0;°C), pH 6–8 (optimum pH 7), and salinity of 0–5.0% (w/v; optimum 1.0%). Strain R7<sup>T</sup> was resistant to cefradine, ampicillin, gentamicin, and spectinomycin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R7<sup>T</sup> belonged to genus <i>Oryzobacter</i>, and showed 98.9% similarity with <i>Oryzobacter terrae</i> PSGM2-16<sup>T</sup>. The average nucleotide identity and digital DNA-DNA hybridization values between strain R7<sup>T</sup> and <i>O. terrae</i> PSGM2-16<sup>T</sup> were 84.5% and 27.6%, respectively. The predominant fatty acids of strain R7<sup>T</sup> were <i>iso</i>-C<sub>16:0</sub>, C<sub>17:1</sub><i>ω</i>8<i>c</i>, <i>iso</i>-C<sub>14:0</sub>, C<sub>17:0</sub>, and <i>iso</i>-C<sub>15:0</sub>. The predominant ubiquinone was MK-8(H<sub>4</sub>). The major polar lipids of strain R7<sup>T</sup> were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, aminophosphoglycolipid, one unidentified phospholipid, and two unidentified polar lipids. The diagnostic diamino acid of the cell-wall peptidoglycan was <i>meso</i>-diaminopimelic acid. Based on the phenotypic, genotypic and phylogenetic evidence, strain R7<sup>T</sup> represents a novel species in the genus <i>Oryzobacter</i>, for which the name <i>Oryzobacter faecalis</i> sp. nov. is proposed. The type strain is R7<sup>T</sup> (=GDMCC 1.4961<sup>T</sup> = KCTC 59403<sup>T</sup> = KACC 23902<sup>T</sup>).</p>

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Oryzobacter faecalis sp. nov., an Antibiotics-Resistant Actinobacterium of the Family Intrasporangiaceae Isolated from Pig Faeces

  • 期刊论文

  • 发表日期：

    2025年6月13日

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  • Xinyan He,
  • Miaohuan Xiong,
  • Bin Liu,
  • Qiuxiang Yan

摘要

A Gram-stain-positive, aerobic, rod-shaped, and non-motile bacterium, designated strain R7T, was isolated from pig faeces. Strain R7T grew at 15–37 °C (optimum 30 °C), pH 6–8 (optimum pH 7), and salinity of 0–5.0% (w/v; optimum 1.0%). Strain R7T was resistant to cefradine, ampicillin, gentamicin, and spectinomycin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R7T belonged to genus Oryzobacter, and showed 98.9% similarity with Oryzobacter terrae PSGM2-16T. The average nucleotide identity and digital DNA-DNA hybridization values between strain R7T and O. terrae PSGM2-16T were 84.5% and 27.6%, respectively. The predominant fatty acids of strain R7T were iso-C16:0, C17:1ω8c, iso-C14:0, C17:0, and iso-C15:0. The predominant ubiquinone was MK-8(H4). The major polar lipids of strain R7T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, aminophosphoglycolipid, one unidentified phospholipid, and two unidentified polar lipids. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on the phenotypic, genotypic and phylogenetic evidence, strain R7T represents a novel species in the genus Oryzobacter, for which the name Oryzobacter faecalis sp. nov. is proposed. The type strain is R7T (=GDMCC 1.4961T = KCTC 59403T = KACC 23902T).