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<p>The screening of FPUase-synthesizing bacteria and optimization of hydrolysis conditions for alkaline pre-treated bagasse are key focuses of current research. This study systematically screened cellulose‑degrading bacteria from soil, mud, and cow liver samples, identifying HL04 as a robust FPUase‑producing strain. Based on 16S rRNA ribosome sequence, HL04 was identified as <i>Bacillus safensis</i>, a newly discovered microorganism capable of synthesizing FPUase. FPUase production conditions were optimized through single‑factor experiments analyzing fermentation time, substrate concentration, pH, and temperature. Using the Box–Behnken Design (BBD) under the response surface methodology (RSM), the study refined these factors to enhance FPUase production. The resulting second-degree polynomial model demonstrated high validity (<i>R</i><sup>2</sup> = 0.9913), and statistically significant interactions (<i>P</i> &lt; 0.05). Optimal conditions—12&#xa0;g/L of substrate concentration, pH 7, and a fermentation temperature of 40&#xa0;°C—yielded the highest FPUase activity of 61.5 U/L.</p>

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Isolation, Screening, Identification of Cellulolytic Bacteria and Optimization of Factors Affecting FPUase Synthesis for Bagasse Hydrolysis

  • 期刊论文

  • 发表日期：

    2025年6月9日

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  • Le Duy Khuong,
  • Van Minh Nguyen,
  • Roan Thi Do,
  • Huong Thi Thanh Doan,
  • Don Nguyen,
  • Sang Van Vu,
  • Hong Thi Nguyen,
  • Ichiro Kamei,
  • Chu Luong Tri

摘要

The screening of FPUase-synthesizing bacteria and optimization of hydrolysis conditions for alkaline pre-treated bagasse are key focuses of current research. This study systematically screened cellulose‑degrading bacteria from soil, mud, and cow liver samples, identifying HL04 as a robust FPUase‑producing strain. Based on 16S rRNA ribosome sequence, HL04 was identified as Bacillus safensis, a newly discovered microorganism capable of synthesizing FPUase. FPUase production conditions were optimized through single‑factor experiments analyzing fermentation time, substrate concentration, pH, and temperature. Using the Box–Behnken Design (BBD) under the response surface methodology (RSM), the study refined these factors to enhance FPUase production. The resulting second-degree polynomial model demonstrated high validity (R2 = 0.9913), and statistically significant interactions (P < 0.05). Optimal conditions—12 g/L of substrate concentration, pH 7, and a fermentation temperature of 40 °C—yielded the highest FPUase activity of 61.5 U/L.