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<p>A Gram-stain-negative, aerobic, short rod-shaped and motile bacterium, designated strain CSK11QG-6<sup>T</sup> was isolated from root of <i>Aegiceras corniculatum</i> taken from Shankou Mangrove Ecological National Nature Reserve in Guangxi Zhuang Autonomous Region, China. Global alignment based on 16S rRNA gene sequences indicated that strain CSK11QG-6<sup>T</sup> shared the highest identity of 96.5% with <i>Consotaella salsifontis</i> USBA 369<sup>T</sup>. Phylogenetic analyses based on 16S rRNA gene sequences and genome sequences indicated that strain CSK11QG-6<sup>T</sup> clustered together with <i>Consotaella salsifontis</i> USBA 369<sup>T</sup>. Strain CSK11QG-6<sup>T</sup> had a genome size of 5.42 Mbp, with a N50 of 97.8&#xa0;kb and coverage of 138 × . The DNA G + C content was 64.8%. The orthologous average nucleotide identity and digital DNA–DNA hybridization values of strain CSK11QG-6<sup>T</sup> with related species fell below the proposed species delineation threshold of 95–96% and 70%, respectively, indicating that strain CSK11QG-6<sup>T</sup> represented a novel species. Strain CSK11QG-6<sup>T</sup> contained C<sub>18:1</sub><i>ω</i>7<i>c</i> and C<sub>16:0</sub> as the predominant fatty acids, and uniquinone-10 as the respiratory quinone. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipids and two unidentified phospholipids. Strain CSK11QG-6<sup>T</sup> exhibited chemical characteristics of fatty acids, lipids and quinones typical for the genus <i>Consotaella</i>, but could be clearly differentiated from <i>Consotaella</i> by many of phenotypic characteristics. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strain CSK11QG-6<sup>T</sup> represents a novel species of the genus <i>Consotaella</i>, for which the name <i>Consotaella aegiceratis</i> sp. nov. is proposed. The type strain is CSK11QG-6<sup>T</sup> (= MCCC 1K07176<sup>T</sup> = JCM 35044<sup>T</sup>).</p>

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Consotaella aegiceratis sp. nov., a Novel Endophytic Bacterium Isolated from Root of Aegiceras corniculatum

  • 期刊论文

  • 发表日期：

    2025年5月27日

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  • Yifen Luo,
  • Chengshao Zan,
  • Mingsheng Chen,
  • Fang Liu,
  • Xiaohui Chen,
  • Zhouqing Zheng,
  • Xiao Ma,
  • Li Tuo

摘要

A Gram-stain-negative, aerobic, short rod-shaped and motile bacterium, designated strain CSK11QG-6T was isolated from root of Aegiceras corniculatum taken from Shankou Mangrove Ecological National Nature Reserve in Guangxi Zhuang Autonomous Region, China. Global alignment based on 16S rRNA gene sequences indicated that strain CSK11QG-6T shared the highest identity of 96.5% with Consotaella salsifontis USBA 369T. Phylogenetic analyses based on 16S rRNA gene sequences and genome sequences indicated that strain CSK11QG-6T clustered together with Consotaella salsifontis USBA 369T. Strain CSK11QG-6T had a genome size of 5.42 Mbp, with a N50 of 97.8 kb and coverage of 138 × . The DNA G + C content was 64.8%. The orthologous average nucleotide identity and digital DNA–DNA hybridization values of strain CSK11QG-6T with related species fell below the proposed species delineation threshold of 95–96% and 70%, respectively, indicating that strain CSK11QG-6T represented a novel species. Strain CSK11QG-6T contained C18:1ω7c and C16:0 as the predominant fatty acids, and uniquinone-10 as the respiratory quinone. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipids and two unidentified phospholipids. Strain CSK11QG-6T exhibited chemical characteristics of fatty acids, lipids and quinones typical for the genus Consotaella, but could be clearly differentiated from Consotaella by many of phenotypic characteristics. Based on phylogenomic and phylogenetic analyses coupled with phenotypic and chemotaxonomic characterizations, strain CSK11QG-6T represents a novel species of the genus Consotaella, for which the name Consotaella aegiceratis sp. nov. is proposed. The type strain is CSK11QG-6T (= MCCC 1K07176T = JCM 35044T).