A novel bacterial strain, designated DFM31T, was isolated from a tidal flat in Gun-San, South Korea and characterized as a novel species within the genus Meridianimarinicoccus. Morphological and biochemical analyses indicated that strain DFM31T was a Gram-stain-negative, coccoid-shaped bacterium without flagella. The strain formed pale orange colonies on MA after 3 days of incubation at 30 °C. The strain was found to survive at 20–37 °C (optimum, 30 °C), pH 6–8 (optimum, pH 7), and 0.5–5% (w/v) NaCl (optimum, 3%). 16S rRNA gene similarity analysis indicated that the strain DFM31T was closely related to the genus Meridianimarinicoccus (95.1–97.5%). Biochemical tests showed that the strain was oxidase and catalase positive. The major fatty acids were Summed Feature 8 [C18:1 <InlineEquation ID="IEq1"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="284_2025_4159_Article_IEq1.gif" Format="GIF" Height="10" Rendition="HTML" Resolution="72" Type="Linedraw" Width="14" /> </InlineMediaObject> <EquationSource Format="TEX">$\omega$</EquationSource> <EquationSource Format="MATHML"><math> <mi>ω</mi> </math></EquationSource> </InlineEquation> 7c/C18:1 <InlineEquation ID="IEq2"> <InlineMediaObject> <ImageObject Color="BlackWhite" FileRef="284_2025_4159_Article_IEq2.gif" Format="GIF" Height="10" Rendition="HTML" Resolution="72" Type="Linedraw" Width="14" /> </InlineMediaObject> <EquationSource Format="TEX">$\omega$</EquationSource> <EquationSource Format="MATHML"><math> <mi>ω</mi> </math></EquationSource> </InlineEquation> 6c] (86.1%) and the major respiratory quinone was Q-10. The DNA G + C % of genomic DNA is 64.4. The values for Average amino acid (AAI), Average nucleotide identity (ANI), and DNA–DNA hybridization (DDH) compared with the related type strains in the genus Meridianimarinicoccus were 70.7–72%, 72.4–75.1%, and 18.7–19.2%, respectively. Based on its distinct phylogenetic, phenotypic, and biochemical characteristics, strain DFM31T is proposed to represent a novel species of the genus Meridianimarinicoccus, for which the name Meridianimarinicoccus marinus sp. nov. is proposed. The type strain is DFM31T (= KEMB 021938 T = KCTC 8650 T = JCM 37289 T).

Meridianimarinicoccus marinus sp. nov., Isolated from Tidal Flat

期刊论文
发表日期：

2025年3月17日

查看全文

Min-Seok Kang,
Jae-Yon Yu,
Hyung-Seop Kim,
Ke Dong,
Sathiyaraj Srinivasan,
Sang-Seob Lee

摘要

A novel bacterial strain, designated DFM31^T, was isolated from a tidal flat in Gun-San, South Korea and characterized as a novel species within the genus Meridianimarinicoccus. Morphological and biochemical analyses indicated that strain DFM31^T was a Gram-stain-negative, coccoid-shaped bacterium without flagella. The strain formed pale orange colonies on MA after 3 days of incubation at 30 °C. The strain was found to survive at 20–37 °C (optimum, 30 °C), pH 6–8 (optimum, pH 7), and 0.5–5% (w/v) NaCl (optimum, 3%). 16S rRNA gene similarity analysis indicated that the strain DFM31^T was closely related to the genus Meridianimarinicoccus (95.1–97.5%). Biochemical tests showed that the strain was oxidase and catalase positive. The major fatty acids were Summed Feature 8 [C_18:1 $\omega$ $ω$ 7c/C_18:1 $\omega$ $ω$ 6c] (86.1%) and the major respiratory quinone was Q-10. The DNA G + C % of genomic DNA is 64.4. The values for Average amino acid (AAI), Average nucleotide identity (ANI), and DNA–DNA hybridization (DDH) compared with the related type strains in the genus Meridianimarinicoccus were 70.7–72%, 72.4–75.1%, and 18.7–19.2%, respectively. Based on its distinct phylogenetic, phenotypic, and biochemical characteristics, strain DFM31^T is proposed to represent a novel species of the genus Meridianimarinicoccus, for which the name Meridianimarinicoccus marinus sp. nov. is proposed. The type strain is DFM31^T (= KEMB 021938^T = KCTC 8650^T = JCM 37289^T).