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<p>Two enterococcal isolates, designated as strains N48-2<sup>T</sup> and N48-9.1, were isolated from traditional Chinese pickle (‘Suan cai’) and characterized using a polyphasic approach. Phylogenies based on 16S rRNA gene sequences and whole genome sequences indicated that strains N48-2<sup>T</sup> and N48-9.1 were most closely related to <i>Enterococcus hermanniensis</i> LMG 12317<sup>T</sup>. Strains N48-2<sup>T</sup> and N48-9.1 shared the highest 16S rRNA (99.5%), <i>pheS</i> (90.3%), <i>rpoA</i> (97.7% and 97.5%) and concatenated <i>pheS</i> and <i>rpoA</i> (95.1% and 95.0%) gene sequence similarities with the type strain of <i>E. hermanniensis</i>. Strains N48-2<sup>T</sup> and N48-9.1 had 86.7%–87.9% ANI, 33.6%–33.8% dDDH and 91.7%–92.2% AAI values with <i>E. hermanniensis</i> DSM 17122<sup>T</sup>. Phenotypically, strains N48-2<sup>T</sup> and N48-9.1 could be distinguished from DSM 17122<sup>T</sup> by acid production from D-galactose, L-sorbose, L-rhamnose and amygdalin, H<sub>2</sub>S production, resistance to streptomycin sulfate (50&#xa0;μg/ml), kanamycin sulfate (50&#xa0;μg/ml), neomycin sulfate (5&#xa0;μg/ml) and gentamicin sulfate (5&#xa0;μg/ml), tolerance to 6% NaCl, and growth at pH 5 and 40&#xa0;°C. Based on these data, strains N48-2<sup>T</sup> (= JCM 37000<sup>T</sup> = CCTCC AB 2024127<sup>T</sup> = LMG 33662<sup>T</sup>) and N48-9.1 represent a novel species, in which the name <i>Enterococcus hailinensis</i> sp. nov., is proposed.</p>

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Enterococcus hailinensis sp. nov., Isolated from Traditional Chinese Pickle

  • 期刊论文

  • 发表日期：

    2025年3月6日

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  • Ting-Yu Wang,
  • Hao Wang,
  • Chun Tao Gu

摘要

Two enterococcal isolates, designated as strains N48-2T and N48-9.1, were isolated from traditional Chinese pickle (‘Suan cai’) and characterized using a polyphasic approach. Phylogenies based on 16S rRNA gene sequences and whole genome sequences indicated that strains N48-2T and N48-9.1 were most closely related to Enterococcus hermanniensis LMG 12317T. Strains N48-2T and N48-9.1 shared the highest 16S rRNA (99.5%), pheS (90.3%), rpoA (97.7% and 97.5%) and concatenated pheS and rpoA (95.1% and 95.0%) gene sequence similarities with the type strain of E. hermanniensis. Strains N48-2T and N48-9.1 had 86.7%–87.9% ANI, 33.6%–33.8% dDDH and 91.7%–92.2% AAI values with E. hermanniensis DSM 17122T. Phenotypically, strains N48-2T and N48-9.1 could be distinguished from DSM 17122T by acid production from D-galactose, L-sorbose, L-rhamnose and amygdalin, H2S production, resistance to streptomycin sulfate (50 μg/ml), kanamycin sulfate (50 μg/ml), neomycin sulfate (5 μg/ml) and gentamicin sulfate (5 μg/ml), tolerance to 6% NaCl, and growth at pH 5 and 40 °C. Based on these data, strains N48-2T (= JCM 37000T = CCTCC AB 2024127T = LMG 33662T) and N48-9.1 represent a novel species, in which the name Enterococcus hailinensis sp. nov., is proposed.