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<p>A Gram-stain positive, long-rod-shaped, motile, and aerobic bacterial strain, designated 16A2E<sup>T</sup>, was isolated from mangrove sediment sample. The 16S rRNA gene sequence similarity analysis indicated that strain 16A2E<sup>T</sup> exhibited high similarity to <i>Ornithinibacillus contaminans</i> CCUG 53201<sup>T</sup> (98.2%), <i>Ornithinibacillus bavariensis</i> WSBC 24001<sup>T</sup> (98.1%), and <i>Ornithinibacillus scapharcae</i> TW25<sup>T</sup> (97.7%). Strain 16A2E<sup>T</sup> grew within a temperature range of 20–50&#xa0;°C&#xa0;(optimum 40&#xa0;°C), a pH range of 6.0–9.0 (optimum pH 8.0), and in the presence of 0–10% (w/v) NaCl (optimum 1%). The genome size of strain 16A2E<sup>T</sup> is 3.60 Mbp, with a G + C content of 36.7%. The overall genome related index (OGRI) analyses revealed low average nucleotide identity (ANI &lt; 75.9%), average amino acid identity (AAI &lt; 77.5%), and digital DNA-DNA hybridization (dDDH &lt; 19.8%) with other species in the genus <i>Ornithinibacillus</i>. Chemotaxonomic analyses revealed that the major polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phospholipids, and an aminophospholipid. The predominant fatty acids were iso-C<sub>15:0</sub>, anteiso-C<sub>15:0</sub>, and anteiso-C<sub>17:0</sub>. The sole respiratory quinone was menaquinone-7 (MK-7), and the peptidoglycan amino acid type was determined to be A4β. Based on phenotypic and genotypic analyses, strain 16A2E<sup>T</sup> (= GDMCC 1.4379<sup>T</sup> = JCM 36753<sup>T</sup>) represents a novel species within the genus <i>Ornithinibacillus</i>, for which the name <i>Ornithinibacillus xuwenensis</i> sp. nov. is proposed.</p>

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Ornithinibacillus xuwenensis sp. nov., A Novel Thermotolerant Bacterium Isolated from Mangrove Sediment

  • 期刊论文

  • 发表日期：

    2025年2月18日

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  • Ming Li,
  • Xixi Hu,
  • Yuan Ni,
  • Tiancheng Ni,
  • Feng Li,
  • Dong Xue

摘要

A Gram-stain positive, long-rod-shaped, motile, and aerobic bacterial strain, designated 16A2ET, was isolated from mangrove sediment sample. The 16S rRNA gene sequence similarity analysis indicated that strain 16A2ET exhibited high similarity to Ornithinibacillus contaminans CCUG 53201T (98.2%), Ornithinibacillus bavariensis WSBC 24001T (98.1%), and Ornithinibacillus scapharcae TW25T (97.7%). Strain 16A2ET grew within a temperature range of 20–50 °C (optimum 40 °C), a pH range of 6.0–9.0 (optimum pH 8.0), and in the presence of 0–10% (w/v) NaCl (optimum 1%). The genome size of strain 16A2ET is 3.60 Mbp, with a G + C content of 36.7%. The overall genome related index (OGRI) analyses revealed low average nucleotide identity (ANI < 75.9%), average amino acid identity (AAI < 77.5%), and digital DNA-DNA hybridization (dDDH < 19.8%) with other species in the genus Ornithinibacillus. Chemotaxonomic analyses revealed that the major polar lipids include diphosphatidylglycerol, phosphatidylglycerol, phospholipids, and an aminophospholipid. The predominant fatty acids were iso-C15:0, anteiso-C15:0, and anteiso-C17:0. The sole respiratory quinone was menaquinone-7 (MK-7), and the peptidoglycan amino acid type was determined to be A4β. Based on phenotypic and genotypic analyses, strain 16A2ET (= GDMCC 1.4379T = JCM 36753T) represents a novel species within the genus Ornithinibacillus, for which the name Ornithinibacillus xuwenensis sp. nov. is proposed.