Proposal of Streptomyces bellus Margalith and Beretta 1960 as a Later Heterotypic Synonym of Streptomyces coeruleorubidus (Preobrazhenskaya 1957) Pridham et al. 1958 and an Emended Description of Streptomyces coeruleorubidus

In the present work, the taxonomic relationship between Streptomyces coeruleorubidus and Streptomyces bellus was reevaluated by a comprehensive comparison of phenotypic, chemotaxonomic and genomic characteristics, as well as phylogeny. In 1957 and 1960, Streptomyces coeruleorubidus and Streptomyces bellus were described as two novel Streptomyces species. The full-length 16S rRNA gene sequence analysis indicated that Streptomyces bellus JCM 4292^T shared highest sequence identity with Streptomyces coerulescens ISP 5146^T (100%). Phylogenetic analysis of 16S rRNA gene sequence showed that S. bellus JCM 4292^T was most closely related to Streptomyces coerulescens ISP 5146^T. Phylogenetic analysis of five housekeeping gene sequences demonstrated that S. bellus JCM 4292^T was most closely related to S. coeruleorubidus ATCC 13740^T. Nevertheless, the ANIm (average nucleotide identity based on MuMmer ultra-rapid aligning tool) and dDDH (digital DNA–DNA hybridization) values between them were 97.71% and 81.9%, respectively, greater than the threshold of 96.7% and 70% for the delineation of Streptomyces species, suggesting that they represent the same genomic species. In addition, phenotypic and chemotaxonomic characteristics, as well as phylogeny and genomic DNA–DNA correlation analysis also confirmed the above conclusion. Consequently, we proposed that S. bellus Margalith and Beretta 1960 is a later heterotypic synonym of S. coeruleorubidus (Preobrazhenskaya 1957) Pridham et al. 1958.