Structural Characterization and Functional Studies of Exopolysaccharide by Native Lacticaseibacillus rhamnosus P14 Isolated from the Moroccan Region

Exopolysaccharides (EPS) are natural polymers synthesized by several microorganisms, including lactic acid bacteria (LAB). They are characterized by a great structural diversity, which gives them interesting biological and pharmacological properties. This work investigates the physicochemical and biological characterization of a new exopolysaccharide (EPS) produced by a wild Lacticaseibacillus rhamnosus P14. The functional groups, chemical bonds, and thermal and morphological properties of the purified EPS-P14 were determined using Fourier Transform Infrared, Nuclear Magnetic Resonance, and X-ray diffraction spectroscopies, as well as Thermo-gravimetric analysis, Differential Scanning Calorimetry and Scanning Electron Microscopy. The functional properties, namely antioxidant and emulsifying activities, were also assessed. The physicochemical analysis revealed that EPS-P14 is a porous and thermally stable polysaccharide with a degradation temperature of 307 °C. NMR and FT-IR studies identified it as a homogeneous α-D-glucan with mainly α-(1 → 6) glycosidic linkage and some α-(1 → 3) branching. EPS-P14 was highly water-soluble and exhibited strong emulsifying and stabilizing properties in a concentration-dependent manner. Furthermore, EPS-P14 demonstrated significant DPPH scavenging and ferric-reducing capacities. These findings suggest that EPS-P14 is a bioactive polysaccharide with potential effects, which could be a promising natural candidate for prospective application.