Scholarlink

<p>To establish age-specific diagnostic cut-off values for HbF and HbA<sub>2</sub> in children under 2 years in Southern Fujian, China, across α-thalassemia group(carriers and patients),β-thalassemia group(carriers), and a negative control group, and evaluate their diagnostic efficacy for precise thalassemia screening.&#xa0;A retrospective analysis was conducted on 1,072 children under 2 years old. Participants were divided into α-thalassemia group(carriers and patients,331 samples), β-thalassemia group(carriers,220 samples), α+β-thalassemia group(10 samples), and negative control groups (511 samples), and further stratified into three age groups: 0–6 months (172 samples), 7–12 months (532 samples), and 13–24 months (368 samples). ROC curve analysis determined optimal cut-off values for HbF and HbA<sub>2</sub>, with AUC, sensitivity, and specificity calculated. Normal reference ranges were also analyzed.&#xa0;In the negative control group, mean HbA<sub>2</sub> and HbF varied significantly with age. For β-thalassemia, optimal HbA<sub>2</sub> cut-offs were 2.6% (0–6 months, AUC=0.849, sensitivity 80.6%, specificity 85.9%), 3.15% (7–12 months, AUC=0.979, sensitivity 97.9%, specificity 98.8%), and 3.7% (13–24 months, AUC=0.990, sensitivity 98.9%, specificity 98.7%). Optimal HbF cut-offs were 10.1% (0–6 months, AUC=0.720), 4.75% (7–12 months, AUC=0.920), and 2.35% (13–24 months, AUC=0.931). For α-thalassemia, HbA<sub>2</sub>cut-offs showed limited diagnostic value (AUC ≤0.667).&#xa0;HbF and HbA<sub>2</sub> levels are significantly influenced by age. Age-stratified cut-off values for HbA<sub>2</sub>demonstrate excellent diagnostic efficacy for β-thalassemia and can serve as primary screening indicators. For α-thalassemia, HbA<sub>2</sub> measurement alone is insufficient, highlighting the need for complementary testing methods to improve diagnostic accuracy.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Analysis of the diagnostic efficacy of hemoglobin capillary electrophoresis for thalassemia in children under 2 years old in Southern Fujian (Minnan) Region, China

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年7月24日

查看全文
  • Xiaolong Liu,
  • Qianmei Zhuang,
  • Meizhen Yan,
  • Chunqiang Liu,
  • Geng Wang,
  • Baojia Huang

摘要

To establish age-specific diagnostic cut-off values for HbF and HbA2 in children under 2 years in Southern Fujian, China, across α-thalassemia group(carriers and patients),β-thalassemia group(carriers), and a negative control group, and evaluate their diagnostic efficacy for precise thalassemia screening. A retrospective analysis was conducted on 1,072 children under 2 years old. Participants were divided into α-thalassemia group(carriers and patients,331 samples), β-thalassemia group(carriers,220 samples), α+β-thalassemia group(10 samples), and negative control groups (511 samples), and further stratified into three age groups: 0–6 months (172 samples), 7–12 months (532 samples), and 13–24 months (368 samples). ROC curve analysis determined optimal cut-off values for HbF and HbA2, with AUC, sensitivity, and specificity calculated. Normal reference ranges were also analyzed. In the negative control group, mean HbA2 and HbF varied significantly with age. For β-thalassemia, optimal HbA2 cut-offs were 2.6% (0–6 months, AUC=0.849, sensitivity 80.6%, specificity 85.9%), 3.15% (7–12 months, AUC=0.979, sensitivity 97.9%, specificity 98.8%), and 3.7% (13–24 months, AUC=0.990, sensitivity 98.9%, specificity 98.7%). Optimal HbF cut-offs were 10.1% (0–6 months, AUC=0.720), 4.75% (7–12 months, AUC=0.920), and 2.35% (13–24 months, AUC=0.931). For α-thalassemia, HbA2cut-offs showed limited diagnostic value (AUC ≤0.667). HbF and HbA2 levels are significantly influenced by age. Age-stratified cut-off values for HbA2demonstrate excellent diagnostic efficacy for β-thalassemia and can serve as primary screening indicators. For α-thalassemia, HbA2 measurement alone is insufficient, highlighting the need for complementary testing methods to improve diagnostic accuracy.