Purpose <p>The inferior temporal septum (ITS) is a fibrous adhesion between the superficial temporal fascia and the superficial layer of the deep temporal fascia. This study comprehensively visualized the three-dimensional (3D) topography of the ITS to provide clinically significant anatomical insights for temporal interventions.</p> Methods <p>Ten temporal specimens were divided into three regions from the anterior direction to the posterior direction to investigate microstructural variations of the ITS according to its location in a histological analysis. Another ten temporal specimens were subjected to a multidirectional analysis of the temporal fasciae using a 3D volume rendering technique.</p> Results <p>The ITS, which continued from the temporal ligamentous adhesion, was composed of thick and short fibrous septa between two temporal fasciae in the anterior temporal region. In the midtemporal region, the fibrous septa of the ITS were relatively thin and long, with abundant adipose tissue. The ITS fibers in the posterior temporal region did not fuse the two temporal fasciae, instead following a course parallel to them.</p> Conclusion <p>This study has revealed microstructural variations of the ITS that depend on its location in the temporal region. The findings of the present study should be considered when performing face-lifting procedures involving the layer under the superficial musculoaponeurotic system, in order to minimize complications such as fascial and neurovascular injuries.</p>