Objective <p>To evaluate the efficacy of autologous fascial adipose tissue transplantation for correcting glabellar frown lines during facial rejuvenation surgery.</p> Methods <p>A retrospective study (2007–2023) analyzed patients with glabellar frown lines undergoing concurrent facial rejuvenation. Fascial adipose strips harvested during rhytidectomy or upper blepharoplasty were subdermally transplanted into glabellar folds. Wrinkle severity was assessed pre- and postoperatively using a validated four-point clinical scale [1], with outcomes evaluated at 3–12 months.</p> Results <p>Eighteen female patients (mean age 52.7 years) showed: (1) 100% improvement (≥1-point reduction) across mild, moderate, and severe wrinkle groups; (2) significant sustained correction of both dynamic and static wrinkles at 12 months; (3) absence of complications (e.g., hematoma, infection); (4) one severe case required adjuvant botulinum toxin for optimal dynamic correction; and (5) outcomes were comparable regardless of fascial tissue harvest site (facial vs. infra-brow; P&gt;0.05).</p> Conclusions <p>Autologous fascial adipose tissue transplantation effectively corrects dynamic and static glabellar wrinkles. This technique leverages discarded tissue, avoids injection-related vascular risks, and serves as a safe adjunct during facial rejuvenation.</p> Level of Evidence IV <p>This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors &#xa0;<a href="http://www.springer.com/00266">www.springer.com/00266</a>.</p>