<p>Although insectivorous bats directly prey on various agricultural pests, their echolocation calls also induce significant anti-predator responses in eared moths. However, the predation risk effects of different ultrasound types of bats on moths especially on crambid moths remain unreported. Here, we exposed the Asian corn borer (ACB) to individual or combined ultrasounds from three insectivorous bat species, with white noise and silent groups serving as controls, to investigate predation risk-induced responses of ACB (from larva to adult) in terms of food intake, female fecundity, amplitude of extracellularly recorded action potentials, and stress-related peroxidase activity. ACB exposed to bat calls exhibited at most 40% reduced food intake and 54% lower fecundity, along with 10-fold higher amplitudes and 22% increased peroxidase activity compared to control. We also found that ultrasound emitted by <i>Hypsugo alaschanicus</i>, whose diet comprised lowest proportion of moths in three species, exerted the most pronounced predation risk effects on ACB. The risk effect of the combination of two types of ultrasounds was higher than that of individual ultrasound. Moreover, female ACB exhibited significantly stronger risk-induced trait responses (including action potentials amplitude of auditory neurons and peroxidase activity level) than males. These results provide experimental evidence that different types of echolocation calls elicit distinct predation risk effects in moths. Notably, even bat species that do not typically prey on moths can trigger strong risk-induced trait responses through their echolocation calls. These findings suggested that bat-derived ultrasounds hold promising potential for application in integrated pest management strategies.</p> Graphical abstract <p></p>