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Abstract <p>The aim of this study was to assess whether a Type VI Secretion System (T6SS) present in the commensal, non-pathogenic <i>Neisseria subflava</i> strain KU1003-01 plays a role in competition between these bacteria and other commensal and pathogenic Gram-negative microorganisms and the yeast <i>Candida albicans</i>. Using an overlap extension PCR strategy, the core T6SS gene encoding membrane protein TssM was knocked out for <i>N. subflava</i> strain KU1003-01. Co-culture competition assays were conducted with either the <i>N. subflava</i> or <i>N. subflava tssM</i> knock-out strains as the attackers and <i>N. gonorrhoeae</i> strain NCCP11945, <i>N. subflava</i> strain KU1003-02, or <i>C. albicans</i> strain 3153 as the targets. There was a significant difference in recoverable target cell CFUs observed following co-culture with the T6SS knock-out strain in comparison to the parent strain. TssM has been identified as critical to T6SS function for a number of species, and our findings indicate that the T6SS in <i>N. subflava</i> contributes to competition with these other species.</p> Key points <p>• <i>Neisseria subflava Type 6 Secretion System is functional</i></p> <p>• <i>Neisseria subflava attacks other microbes of the same niche</i></p> <p>• <i>Neisseria subflava is competent for transformation</i></p>

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Neisseria subflava Type 6 Secretion System competition with bacterial and fungal species

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月23日

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  • Alan Calder,
  • Lori A. S. Snyder

摘要

Abstract

The aim of this study was to assess whether a Type VI Secretion System (T6SS) present in the commensal, non-pathogenic Neisseria subflava strain KU1003-01 plays a role in competition between these bacteria and other commensal and pathogenic Gram-negative microorganisms and the yeast Candida albicans. Using an overlap extension PCR strategy, the core T6SS gene encoding membrane protein TssM was knocked out for N. subflava strain KU1003-01. Co-culture competition assays were conducted with either the N. subflava or N. subflava tssM knock-out strains as the attackers and N. gonorrhoeae strain NCCP11945, N. subflava strain KU1003-02, or C. albicans strain 3153 as the targets. There was a significant difference in recoverable target cell CFUs observed following co-culture with the T6SS knock-out strain in comparison to the parent strain. TssM has been identified as critical to T6SS function for a number of species, and our findings indicate that the T6SS in N. subflava contributes to competition with these other species.

Key points

• Neisseria subflava Type 6 Secretion System is functional

• Neisseria subflava attacks other microbes of the same niche

• Neisseria subflava is competent for transformation