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<p>The proliferation of antibiotic resistance genes (ARGs) represents a critical global health threat, with wastewater treatment plants (WWTPs) identified as major hotspots for resistance amplification and dissemination. While bacterial conjugation has been extensively studied, bacteriophages are emerging as significant but underestimated vectors for ARG transfer via transduction. We hypothesize that phages are capable of withstanding disinfection and exploiting diverse bacterial hosts in receiving environments, thereby functioning as active vectors of ARG dissemination rather than passive genetic reservoirs. Meta-analysis of disinfection efficacy data (<i>n</i> = 79 experimental observations, 6 technology categories) reveals significant differences in log₁₀ reduction across disinfection technologies (Kruskal–Wallis: <i>H</i> = 25.96, df = 5, <i>p</i> &lt; 0.0001). Ozonation was significantly more effective than chlorination (<i>p</i> &lt; 0.001), membrane processes (<i>p</i> = 0.003), UV irradiation (<i>p</i> = 0.005), and PAA/thermal treatment (<i>p</i> = 0.010). Phage persistence in receiving waters follows a 1–4&#xa0;week timeline modulated by temperature (Q₁₀ ~ 2–3), solar UV, and water chemistry. Broad host range phages have the potential to transfer ARGs to environmentally dominant genera (<i>Aeromonas</i>,<i> Pseudomonas</i>,<i> Vibrio</i>), and sub-inhibitory antibiotics may induce prophages and enhance bacterial competence, creating synergistic conditions that could accelerate resistome evolution. This review underscores the need for integrated approaches combining phage-targeted disinfection with ecological monitoring to effectively restrain the spread of antibiotic resistance.</p> Graphical abstract <p>Bacteriophage-mediated dissemination of antibiotic resistance genes (ARGs) from wastewater treatment plants (WWTPs) to receiving environments. Phages acquire ARGs within WWTPs, survive disinfection treatment, and are released via effluent into receiving waters where they persist for 1–4&#xa0;weeks. Broad host range phages subsequently transfer clinically significant ARGs including <i>blaTEM</i>,<i> sul1</i>, and<i> intI1</i> to diverse environmental bacterial hosts via transduction, facilitating resistome evolution independent of bacterial survival.</p> <p></p>

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Bacteriophage survival and ARG dissemination from wastewater treatment plants to the environment

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月27日

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  • Syeda Fazoon Kazmi,
  • Fang Ru,
  • Yinghua Zhang,
  • Jun Feng,
  • Dong Wu

摘要

The proliferation of antibiotic resistance genes (ARGs) represents a critical global health threat, with wastewater treatment plants (WWTPs) identified as major hotspots for resistance amplification and dissemination. While bacterial conjugation has been extensively studied, bacteriophages are emerging as significant but underestimated vectors for ARG transfer via transduction. We hypothesize that phages are capable of withstanding disinfection and exploiting diverse bacterial hosts in receiving environments, thereby functioning as active vectors of ARG dissemination rather than passive genetic reservoirs. Meta-analysis of disinfection efficacy data (n = 79 experimental observations, 6 technology categories) reveals significant differences in log₁₀ reduction across disinfection technologies (Kruskal–Wallis: H = 25.96, df = 5, p < 0.0001). Ozonation was significantly more effective than chlorination (p < 0.001), membrane processes (p = 0.003), UV irradiation (p = 0.005), and PAA/thermal treatment (p = 0.010). Phage persistence in receiving waters follows a 1–4 week timeline modulated by temperature (Q₁₀ ~ 2–3), solar UV, and water chemistry. Broad host range phages have the potential to transfer ARGs to environmentally dominant genera (Aeromonas, Pseudomonas, Vibrio), and sub-inhibitory antibiotics may induce prophages and enhance bacterial competence, creating synergistic conditions that could accelerate resistome evolution. This review underscores the need for integrated approaches combining phage-targeted disinfection with ecological monitoring to effectively restrain the spread of antibiotic resistance.

Graphical abstract

Bacteriophage-mediated dissemination of antibiotic resistance genes (ARGs) from wastewater treatment plants (WWTPs) to receiving environments. Phages acquire ARGs within WWTPs, survive disinfection treatment, and are released via effluent into receiving waters where they persist for 1–4 weeks. Broad host range phages subsequently transfer clinically significant ARGs including blaTEM, sul1, and intI1 to diverse environmental bacterial hosts via transduction, facilitating resistome evolution independent of bacterial survival.