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Abstract <p>Microbial production of carotenoids provides a sustainable alternative to plant-derived sources. In this study, a yeast strain, <i>Rhodotorula mucilaginosa</i> LB-01, was isolated and its carotenoid biosynthetic potential was evaluated. Medium optimization (10&#xa0;g/L glucose and 3&#xa0;g/L yeast extract) improved relative carotenoid accumulation, reaching 10.13 Abs/g dry biomass. The influence of light quality was subsequently assessed, showing that blue LED illumination was associated with higher relative carotenoid accumulation (12.4 Abs/g) compared with red, orange, white, and green light conditions. Cultivation in a stirred-tank bioreactor (2.5 L working volume) under blue LED illumination resulted in further increases in carotenoid accumulation, reaching 16 Abs/g and 311&#xa0;µg/g dry biomass, corresponding to an approximately 20% increase compared with control conditions operated without direct light exposure. Purification and quantification of individual pigments identified torularhodin (173&#xa0;µg/g), torulene (71&#xa0;µg/g), and β-carotene (67&#xa0;µg/g), all present at higher levels than in the control. Overall, these results indicate that <i>R. mucilaginosa</i> LB-01, combined with controlled LED illumination, represents a promising microbial system for carotenoid accumulation.</p> Key points <p>• <i>Rhodotorula mucilaginosa LB-01 was identified as a carotenoid-producing yeast.</i></p> <p>• <i>Blue LED illumination yielded the highest relative carotenoid accumulation (12.4 Abs/g).</i></p> <p>• <i>Blue LED bioreactor cultivation increased accumulation to 16 Abs/g dry biomass (+ 20%).</i></p>

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Blue LED–assisted bioreactor cultivation enhances carotenoid accumulation in Rhodotorula mucilaginosa LB-01

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月24日

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  • Aldana L. Delgado Cuadros,
  • Nayeli Ortiz Silos,
  • Kiara Garcia Bustos,
  • Kevin Tejada Meza,
  • Gilberto Colina Andrade,
  • Ruly Terán Hilares

摘要

Abstract

Microbial production of carotenoids provides a sustainable alternative to plant-derived sources. In this study, a yeast strain, Rhodotorula mucilaginosa LB-01, was isolated and its carotenoid biosynthetic potential was evaluated. Medium optimization (10 g/L glucose and 3 g/L yeast extract) improved relative carotenoid accumulation, reaching 10.13 Abs/g dry biomass. The influence of light quality was subsequently assessed, showing that blue LED illumination was associated with higher relative carotenoid accumulation (12.4 Abs/g) compared with red, orange, white, and green light conditions. Cultivation in a stirred-tank bioreactor (2.5 L working volume) under blue LED illumination resulted in further increases in carotenoid accumulation, reaching 16 Abs/g and 311 µg/g dry biomass, corresponding to an approximately 20% increase compared with control conditions operated without direct light exposure. Purification and quantification of individual pigments identified torularhodin (173 µg/g), torulene (71 µg/g), and β-carotene (67 µg/g), all present at higher levels than in the control. Overall, these results indicate that R. mucilaginosa LB-01, combined with controlled LED illumination, represents a promising microbial system for carotenoid accumulation.

Key points

• Rhodotorula mucilaginosa LB-01 was identified as a carotenoid-producing yeast.

• Blue LED illumination yielded the highest relative carotenoid accumulation (12.4 Abs/g).

• Blue LED bioreactor cultivation increased accumulation to 16 Abs/g dry biomass (+ 20%).