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Abstract <p>Poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) polymers are accumulated by diverse prokaryotes. Their distinct monomer compositions enable their use as tailored bioplastics. The aims were to characterize the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) synthesis by <i>Paraburkholderia xenovorans</i> LB400 using different sugars and valerate, and to gain genome-oriented insights into polyhydroxyalkanoate production. d-Glucose, d-mannitol, d-gluconate, and d-xylose were evaluated as sole carbon sources or supplemented with valerate. Polyhydroxyalkanoates synthesized by strain LB400 were characterized through GC–MS, GC-FID, FTIR, and <sup>1</sup>H and <sup>13</sup>C-NMR. <i>P. xenovorans</i> LB400 reached 1.00–1.39&#xa0;g L<sup>−1</sup> of dry cell weight (DCW) with a P(3HB) content of 21–43% w w<sup>−1</sup> when grown on different sugars. The addition of valerate to the sugar-grown LB400 cultures yielded a DCW of 1.79 to 2.29&#xa0;g L<sup>−1</sup> and a P(3HB-<i>co</i>-3HV) content of 50.0‒51.2% w w<sup>−1</sup>, with varying 3HV compositions (28‒43&#xa0;mol%). The highest 3HV incorporation was observed with d-xylose and valerate. Genomic analyses of strain LB400 revealed key elements of sugar metabolism influencing growth, polymer accumulation, and monomer composition. LB400 genome encodes the PhaJ-like <i>R</i>-specific hydratase and FadJ epimerase, which are potentially useful for modulating copolymer composition. PHA production under bioreactor conditions was evaluated. In a bioreactor fed with d-glucose, LB400 achieved a P(3HB) concentration of 2.2&#xa0;g L<sup>−1</sup>. These findings highlight the metabolic versatility of <i>P. xenovorans</i> LB400 in utilizing diverse sugars to produce either P(3HB) or tailor-made P(3HB<i>-co</i>-3HV)<i>,</i> supporting the development of bioplastics for specific applications.</p> Key points <p>• <i>Strain LB400 produced P(3HB-co-3HV) from various sugars and valerate.</i></p> <p>• <i>Sugar type drives LB400 PHA copolymer synthesis and composition.</i></p> <p>• <i>Strain LB400 PHA production was scaled up to a bioreactor.</i></p>

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Versatile sugar and valerate metabolic pathways in Paraburkholderia xenovorans LB400 enable tailored poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年10月29日

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  • Mario I. Sepúlveda,
  • Viviana Urtuvia,
  • Natalia Álvarez-Santullano,
  • Pamela Villegas,
  • Jacqueline Vásquez-Navarrete,
  • Valentina Saffirio,
  • Alvaro Díaz-Barrera,
  • Myriam González,
  • Jose Gregório C. Gomez,
  • Valentina Méndez,
  • Michael Seeger

摘要

Abstract

Poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) polymers are accumulated by diverse prokaryotes. Their distinct monomer compositions enable their use as tailored bioplastics. The aims were to characterize the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) synthesis by Paraburkholderia xenovorans LB400 using different sugars and valerate, and to gain genome-oriented insights into polyhydroxyalkanoate production. d-Glucose, d-mannitol, d-gluconate, and d-xylose were evaluated as sole carbon sources or supplemented with valerate. Polyhydroxyalkanoates synthesized by strain LB400 were characterized through GC–MS, GC-FID, FTIR, and 1H and 13C-NMR. P. xenovorans LB400 reached 1.00–1.39 g L−1 of dry cell weight (DCW) with a P(3HB) content of 21–43% w w−1 when grown on different sugars. The addition of valerate to the sugar-grown LB400 cultures yielded a DCW of 1.79 to 2.29 g L−1 and a P(3HB-co-3HV) content of 50.0‒51.2% w w−1, with varying 3HV compositions (28‒43 mol%). The highest 3HV incorporation was observed with d-xylose and valerate. Genomic analyses of strain LB400 revealed key elements of sugar metabolism influencing growth, polymer accumulation, and monomer composition. LB400 genome encodes the PhaJ-like R-specific hydratase and FadJ epimerase, which are potentially useful for modulating copolymer composition. PHA production under bioreactor conditions was evaluated. In a bioreactor fed with d-glucose, LB400 achieved a P(3HB) concentration of 2.2 g L−1. These findings highlight the metabolic versatility of P. xenovorans LB400 in utilizing diverse sugars to produce either P(3HB) or tailor-made P(3HB-co-3HV), supporting the development of bioplastics for specific applications.

Key points

• Strain LB400 produced P(3HB-co-3HV) from various sugars and valerate.

• Sugar type drives LB400 PHA copolymer synthesis and composition.

• Strain LB400 PHA production was scaled up to a bioreactor.