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Abstract <p>Biological flocculation is a complex phenomenon that is often treated as a black box. As a result, flocculation problems are usually remediated without knowledge of the exact causes. We show that it is feasible to exploit a model (N-cycle) consortium with reduced complexity to fundamentally study bioflocculation. Strong nitrifier microcolonies were formed during oxic/anoxic cycles in sequencing batch reactors, using alginate entrapment as a cell retention system. After the release of these aggregates into suspension, macroclusters with flocs of the denitrifier were observed. These results suggest that a living model of a full-scale activated sludge floc can be built through the use of this bottom-up approach. By eliminating shifts in the microbial community, the applied experimental conditions have a more direct effect on the observations. Key Points<InlineEquation ID="IEq1"> <EquationSource Format="TEX">\(\bullet \)</EquationSource> <EquationSource Format="MATHML"><math> <mo>∙</mo> </math></EquationSource> </InlineEquation> <i>Studying flocculation with a model consortium is feasible</i> <InlineEquation ID="IEq2"> <EquationSource Format="TEX">\(\bullet \)</EquationSource> <EquationSource Format="MATHML"><math> <mo>∙</mo> </math></EquationSource> </InlineEquation> <i>Alginate entrapment leads to strong microcolony formation of nitrifiers</i> <InlineEquation ID="IEq3"> <EquationSource Format="TEX">\(\bullet \)</EquationSource> <EquationSource Format="MATHML"><math> <mo>∙</mo> </math></EquationSource> </InlineEquation> <i>FISH by itself is not suitable to study aggregation of a coculture</i></p> Graphic abstract <p></p>

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Establishing a co-culture aggregate of N-cycle bacteria to elucidate flocculation in biological wastewater treatment

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月19日

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  • Laurens Parret,
  • Kenneth Simoens,
  • Benjamin Horemans,
  • Jo De Vrieze,
  • Ilse Smets

摘要

Abstract

Biological flocculation is a complex phenomenon that is often treated as a black box. As a result, flocculation problems are usually remediated without knowledge of the exact causes. We show that it is feasible to exploit a model (N-cycle) consortium with reduced complexity to fundamentally study bioflocculation. Strong nitrifier microcolonies were formed during oxic/anoxic cycles in sequencing batch reactors, using alginate entrapment as a cell retention system. After the release of these aggregates into suspension, macroclusters with flocs of the denitrifier were observed. These results suggest that a living model of a full-scale activated sludge floc can be built through the use of this bottom-up approach. By eliminating shifts in the microbial community, the applied experimental conditions have a more direct effect on the observations. Key Points \(\bullet \) ∙ Studying flocculation with a model consortium is feasible \(\bullet \) ∙ Alginate entrapment leads to strong microcolony formation of nitrifiers \(\bullet \) ∙ FISH by itself is not suitable to study aggregation of a coculture

Graphic abstract