Abstract <p>The overuse of surface disinfectants has been shown to lead to increased microbial resistance, often due to their incorrect application. The aim of this work was to develop and investigate the use of an indicative detection kit for hydrogen peroxide-based disinfectants on porous and non-porous surfaces. The detection kit allows for the detection of the disinfectant up to 7 days after its application, resulting in a colour change from transparent to yellow-orange on both on porous and non-porous surfaces. Concurrent antimicrobial tests were performed to establish the correlation between the colour change and the retention of the disinfectant on the surfaces, thereby confirming the maintenance of its antimicrobial efficacy.</p> Key points <p><UnorderedList Mark="Bullet"> <ItemContent> <p><i>Increased microbial resistance often results from overuse of disinfectants.</i></p> </ItemContent> <ItemContent> <p><i>CUsKit is a hydrogen peroxide-based disinfectant detection kit.</i></p> </ItemContent> <ItemContent> <p><i>CUsKit is an excellent option to prove the presence of disinfectants.</i></p> </ItemContent> </UnorderedList></p>