Abstract <p>Second-generation (2G) bioethanol production, derived from lignocellulosic biomass, has emerged as a sustainable alternative to fossil fuels by addressing growing energy demands and environmental concerns. Fungal sugar transporters (STs) play a critical role in this process, enabling the uptake of monosaccharides such as glucose and xylose, which are released during the enzymatic hydrolysis of biomass. This mini-review explores recent advances in the structural and functional characterization of STs in filamentous fungi and yeasts, highlighting their roles in processes such as cellulase induction, carbon catabolite repression, and sugar signaling pathways. The review also emphasizes the potential of genetic engineering to enhance the specificity and efficiency of these transporters, overcoming challenges such as substrate competition and limited pentose metabolism in industrial strains. By integrating the latest research findings, this work underscores the pivotal role of fungal STs in optimizing lignocellulosic bioethanol production and advancing the bioeconomy. Future prospects for engineering transport systems and their implications for industrial biotechnology are also discussed.</p> Key points <p><UnorderedList Mark="Bullet"> <ItemContent> <p>STs present a conserved structure with different sugar affinities</p> </ItemContent> <ItemContent> <p>STs are involved in the signaling and transport of sugars derived from plant biomass</p> </ItemContent> <ItemContent> <p>Genetic engineering of STs can improve 2G bioethanol production</p> </ItemContent> </UnorderedList></p>