Scholarlink

Background <p>Dermal fibroblast heterogeneity in pigs has long been recognized, which showed great necessity to pay more attention to the potential influence of proliferative heterogeneity on fibrogenic heterogeneity after prolonged in vitro culture. The objective of this study was to explore the effect of the lower capacity of proliferation of dermal fibroblasts from deep porcine dermis on its higher capacity of secreting collagen and to introduce a WST-8-based method to determine the secretion function of cells cultured in vitro.</p> Methods <p>Superficial and deep dermal fibroblasts were cultured from the dorsa of 3 female red Duroc pigs. Superficial and deep dermal fibroblasts from passage 5 were equally seeded in 6-well plates and cultured for 9 days. The collagen secreted by each group was determined using a hydroxyproline assay kit at 5 d, 7 d, 8 d, and 9 d. The cell numbers of each group were determined using the WST-8 assay at 12&#xa0;h, 5 d, and 9 d. At 9 d, the cell cycle distribution of each group was analyzed by flow cytometry.</p> Results <p>At 5 d, the collagen secreted by the deep dermal fibroblasts was significantly higher than that secreted by the superficial dermal fibroblasts. However, the content of collagen of the two groups exhibited no significant difference at times of 7 d, 8 d, and 9 d. The cell numbers of the two groups exhibited no significant difference at the time of 12&#xa0;h, but exhibited significant difference at times of 5 d and 9 d. The content of collagen of the deep dermal fibroblasts modified by the cell numbers was significantly higher than that of the superficial fibroblasts at times of 5 d and 9 d. At the time of 9 d, deep dermal fibroblasts exhibited significantly lower G2 + S percentage and S percentage than the superficial fibroblasts.</p> Conclusion <p>This study showed that the proliferative heterogeneity of dermal fibroblasts can affect their fibrogenic heterogeneity after prolonged in vitro culture and showed great feasibility using WST-8-based method for determining the secretion function of cells cultured in vitro.</p> Level of evidence <p>Not gradable.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The proliferative heterogeneity of dermal fibroblasts can affect their fibrogenic heterogeneity after prolonged in vitro culture

  • 期刊论文

  • 发表日期：

    2025年12月19日

查看全文
  • Yanhai Zuo,
  • Aijun Chen

摘要

Background

Dermal fibroblast heterogeneity in pigs has long been recognized, which showed great necessity to pay more attention to the potential influence of proliferative heterogeneity on fibrogenic heterogeneity after prolonged in vitro culture. The objective of this study was to explore the effect of the lower capacity of proliferation of dermal fibroblasts from deep porcine dermis on its higher capacity of secreting collagen and to introduce a WST-8-based method to determine the secretion function of cells cultured in vitro.

Methods

Superficial and deep dermal fibroblasts were cultured from the dorsa of 3 female red Duroc pigs. Superficial and deep dermal fibroblasts from passage 5 were equally seeded in 6-well plates and cultured for 9 days. The collagen secreted by each group was determined using a hydroxyproline assay kit at 5 d, 7 d, 8 d, and 9 d. The cell numbers of each group were determined using the WST-8 assay at 12 h, 5 d, and 9 d. At 9 d, the cell cycle distribution of each group was analyzed by flow cytometry.

Results

At 5 d, the collagen secreted by the deep dermal fibroblasts was significantly higher than that secreted by the superficial dermal fibroblasts. However, the content of collagen of the two groups exhibited no significant difference at times of 7 d, 8 d, and 9 d. The cell numbers of the two groups exhibited no significant difference at the time of 12 h, but exhibited significant difference at times of 5 d and 9 d. The content of collagen of the deep dermal fibroblasts modified by the cell numbers was significantly higher than that of the superficial fibroblasts at times of 5 d and 9 d. At the time of 9 d, deep dermal fibroblasts exhibited significantly lower G2 + S percentage and S percentage than the superficial fibroblasts.

Conclusion

This study showed that the proliferative heterogeneity of dermal fibroblasts can affect their fibrogenic heterogeneity after prolonged in vitro culture and showed great feasibility using WST-8-based method for determining the secretion function of cells cultured in vitro.

Level of evidence

Not gradable.