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<p>Temperature-based strategies are commonly applied to control microbial growth and mitigate the deleterious effects of biofilms on water systems. However, the impact of these thermal procedures on biofilm structure and stability is usually not evaluated. This study addresses specific aspects of the biofilm’s structure after being exposed to elevated temperatures. Eight-day-old <i>Pseudomonas fluorescens</i> biofilms were formed in a Center for Disease Control (CDC) biofilm reactor under two distinct shear stresses and exposed to 70ºC for 15&#xa0;min. The biofilm structural features were evaluated 1&#xa0;h and 24&#xa0;h after disinfection. Biofilm three-dimensional (3D) mesoscale structural characteristics were analyzed through Optical Coherence Tomography (OCT). Biofilms formed under both shear stresses were partially removed after the temperature shock and suffered structural rearrangements over 1&#xa0;h and 24&#xa0;h. Both biofilms seem to rearrange after 24&#xa0;h into structures that combine enhanced compactness with increased porosity. The present work provides a methodology to address future studies concerning the impact of temperature increase on biofilm structural aspects.</p>

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How biofilm history affects the impact of thermal disinfection on biofilm control and regrowth

  • 期刊论文

  • 发表日期：

    2025年8月7日

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  • Ana Rosa Silva,
  • Luís F. Melo,
  • Ana Pereira

摘要

Temperature-based strategies are commonly applied to control microbial growth and mitigate the deleterious effects of biofilms on water systems. However, the impact of these thermal procedures on biofilm structure and stability is usually not evaluated. This study addresses specific aspects of the biofilm’s structure after being exposed to elevated temperatures. Eight-day-old Pseudomonas fluorescens biofilms were formed in a Center for Disease Control (CDC) biofilm reactor under two distinct shear stresses and exposed to 70ºC for 15 min. The biofilm structural features were evaluated 1 h and 24 h after disinfection. Biofilm three-dimensional (3D) mesoscale structural characteristics were analyzed through Optical Coherence Tomography (OCT). Biofilms formed under both shear stresses were partially removed after the temperature shock and suffered structural rearrangements over 1 h and 24 h. Both biofilms seem to rearrange after 24 h into structures that combine enhanced compactness with increased porosity. The present work provides a methodology to address future studies concerning the impact of temperature increase on biofilm structural aspects.