<p>Douglas-fir (<i>Pseudotsuga menziesii</i>) is the principal species for lumber production in the Pacific northwest and has been the subject of ongoing tree improvement and silvicultural research aimed at enhancing growth. With industry shifting to lower planting densities, it is critical that we improve our knowledge of the wood quality implications of these practices. Hyperspectral imaging (HSI) presents a promising tool for studying within-tree variation of wood properties and it was utilized to provide data for the development of maps illustrating within-tree variation of physicomechanical, tracheid and chemical properties of 25-year-old Douglas-fir grown at different spacings (narrow, conventional and wide). Property maps showed that the different spacings had similar patterns with a more abrupt change in wood properties radially in narrowly spaced trees compared to those widely spaced, regardless of the property assessed. Radial variation patterns showed increases from pith to bark in density, stiffness, tracheid length and width, and glucose/cellulose content, while microfibril angle, lignin, and xylose decreased. Although planting density may not determine absolute wood quality traits, it can influence their developmental patterns across the stem.</p>