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<p>The present study characterizes honey from seven stingless bee species from Mexico, performing physicochemical analysis, melissopalynology, and laser-induced fluorescence photometry imaging. The last is applied as a proof of principle and is a non-destructive method, which requires a tiny amount of sample that fits honey from low-production species, such as these. The use of fluorescence photometry imaging and multivariate analysis allows for the determination of a floral tendency among the bee honey collected for this study. Considering all the aforementioned analyses, it was possible to contribute to establishing a quality control standard for honey from these bees. The results reveal moisture levels ranging from 22% to even higher values, exceeding 30% in some cases. All samples show a pH smaller than 5, which favors the generation of Hydroxymethylfurfural (HMF). Besides, HMF in all the studied samples remains below 8&#xa0;mg/100&#xa0;g, which is the maximum value specified in the Codex. From the results, it is observed that HMF levels are not directly correlated with the honey color (Pfund) or the fluorescence signal. A multivariate analysis is presented for the luminance intensity profiles and the fluorescence color channels, which describe a tendency towards the mono or multifloral nature of the samples, and a relation between the colorimetric data and the altitude where the honey was collected, respectively. Information obtained from this proposed spectroscopic methodology provides new insights into the characteristics of honey from these yet understudied species of stingless bees.</p>

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Fluorescence photometry imaging with physicochemical analysis in seven different southeast Mexico stingless bee honey

  • 期刊论文

  • 发表日期：

    2026年5月12日

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  • Isabel May-Canché,
  • Martín Ortiz-Morales,
  • Manuel H. De la Torre-Ibarra,
  • Rémy Vandame,
  • Claudio Frausto-Reyes

摘要

The present study characterizes honey from seven stingless bee species from Mexico, performing physicochemical analysis, melissopalynology, and laser-induced fluorescence photometry imaging. The last is applied as a proof of principle and is a non-destructive method, which requires a tiny amount of sample that fits honey from low-production species, such as these. The use of fluorescence photometry imaging and multivariate analysis allows for the determination of a floral tendency among the bee honey collected for this study. Considering all the aforementioned analyses, it was possible to contribute to establishing a quality control standard for honey from these bees. The results reveal moisture levels ranging from 22% to even higher values, exceeding 30% in some cases. All samples show a pH smaller than 5, which favors the generation of Hydroxymethylfurfural (HMF). Besides, HMF in all the studied samples remains below 8 mg/100 g, which is the maximum value specified in the Codex. From the results, it is observed that HMF levels are not directly correlated with the honey color (Pfund) or the fluorescence signal. A multivariate analysis is presented for the luminance intensity profiles and the fluorescence color channels, which describe a tendency towards the mono or multifloral nature of the samples, and a relation between the colorimetric data and the altitude where the honey was collected, respectively. Information obtained from this proposed spectroscopic methodology provides new insights into the characteristics of honey from these yet understudied species of stingless bees.