<p>Seafood mislabeling remains a persistent issue globally, despite the implementation of regulatory frameworks at both national and European levels. In Greece, crustaceans—particularly shrimp—are highly valued by consumers and widely available across retail sectors. However, common processing and marketing practices often hinder identification, and the limited public awareness of labeling regulations further contribute to misidentification. This study is the first to apply DNA barcoding to evaluate the extent of mislabeling in the crustacean market of Greece. Two mitochondrial markers, cytochrome c oxidase subunit I (COI) and 16&#xa0;S ribosomal RNA (16&#xa0;S), were employed to identify species from 140 samples collected across four major Greek cities. At least 20 species were identified, and 43 samples (31.38%) were found to be mislabeled. Over half of the species identified were non-native to the Mediterranean Sea, and the majority of samples (66.92%) were imported. The whiteleg shrimp (<i>Penaeus (Litopenaeus) vannamei (Boone</i>,<i> 1931))</i> was the most frequently detected and most commonly mislabeled species, often under generic names that may suggest a local origin. These findings highlight ongoing challenges in labeling transparency and traceability while contributing to the broader understanding of seafood fraud in the region.</p>

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Cracking the shell: investigating crustaceans in the Greek market using DNA barcoding

  • Zoi Giagkazoglou,
  • Loukovitis Dimitrios,
  • Chrysoula Gubili,
  • Dimitrios Chatziplis,
  • Zissis Tzikas,
  • Anastasia Imsiridou

摘要

Seafood mislabeling remains a persistent issue globally, despite the implementation of regulatory frameworks at both national and European levels. In Greece, crustaceans—particularly shrimp—are highly valued by consumers and widely available across retail sectors. However, common processing and marketing practices often hinder identification, and the limited public awareness of labeling regulations further contribute to misidentification. This study is the first to apply DNA barcoding to evaluate the extent of mislabeling in the crustacean market of Greece. Two mitochondrial markers, cytochrome c oxidase subunit I (COI) and 16 S ribosomal RNA (16 S), were employed to identify species from 140 samples collected across four major Greek cities. At least 20 species were identified, and 43 samples (31.38%) were found to be mislabeled. Over half of the species identified were non-native to the Mediterranean Sea, and the majority of samples (66.92%) were imported. The whiteleg shrimp (Penaeus (Litopenaeus) vannamei (Boone, 1931)) was the most frequently detected and most commonly mislabeled species, often under generic names that may suggest a local origin. These findings highlight ongoing challenges in labeling transparency and traceability while contributing to the broader understanding of seafood fraud in the region.