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<p>Peony seed meal is the residue obtained after oil extraction from peony seeds, known for its low bioavailability. However, it may possess significant nutritional value and bioactivity, indicating notable antioxidant activity. Nonetheless, research on its antioxidant mechanisms is currently lacking. This study investigated the antioxidant activity of peony seed protein hydrolysates, identifying four antioxidant peptides through LC-MS/MS and peptidomics. The selected antioxidant peptides were screened for their effects on the Keap1-Nrf2 pathway through molecular docking simulations. Subsequently, two peptides, LPSHPPR and LHPHFR, were chosen for cellular antioxidant experiments, demonstrating their ability to reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levels and confer protection against H<sub>2</sub>O<sub>2</sub>-induced RAW264.7 cell damage. Furthermore, we validated the regulatory role of LPSHPPR and LHPHFR in the Keap1-Nrf2 pathway, inducing the overexpression of antioxidant enzymes such as heme oxygenase 1 (HO-1). Molecular docking and western blot analyses suggested that the potential molecular mechanisms of LPSHPPR and LHPHFR may involve occupying the Nrf2 binding site on Keap1 or disrupting the interaction between Keap1 and Nrf2, thereby activating the Keap1-Nrf2 pathway. Overall, the study provides a theoretical basis for the application of peony seed antioxidant peptides in functional foods and offers new insights into the identification and mechanistic studies of more food-derived antioxidant peptides.</p>

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Discovery of novel antioxidant peptides from peony seed protein hydrolysates through in vivo studies and in silico

  • 期刊论文

  • 发表日期：

    2025年3月10日

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  • Huatian Wang,
  • Zhou Yang,
  • Zhen Cao,
  • Tao Feng,
  • Lingyun Yao,
  • Min Sun,
  • Shiqing Song,
  • Qian Liu,
  • Chuang Yu

摘要

Peony seed meal is the residue obtained after oil extraction from peony seeds, known for its low bioavailability. However, it may possess significant nutritional value and bioactivity, indicating notable antioxidant activity. Nonetheless, research on its antioxidant mechanisms is currently lacking. This study investigated the antioxidant activity of peony seed protein hydrolysates, identifying four antioxidant peptides through LC-MS/MS and peptidomics. The selected antioxidant peptides were screened for their effects on the Keap1-Nrf2 pathway through molecular docking simulations. Subsequently, two peptides, LPSHPPR and LHPHFR, were chosen for cellular antioxidant experiments, demonstrating their ability to reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levels and confer protection against H2O2-induced RAW264.7 cell damage. Furthermore, we validated the regulatory role of LPSHPPR and LHPHFR in the Keap1-Nrf2 pathway, inducing the overexpression of antioxidant enzymes such as heme oxygenase 1 (HO-1). Molecular docking and western blot analyses suggested that the potential molecular mechanisms of LPSHPPR and LHPHFR may involve occupying the Nrf2 binding site on Keap1 or disrupting the interaction between Keap1 and Nrf2, thereby activating the Keap1-Nrf2 pathway. Overall, the study provides a theoretical basis for the application of peony seed antioxidant peptides in functional foods and offers new insights into the identification and mechanistic studies of more food-derived antioxidant peptides.