Scholarlink

<p>The term ‘wild’ is a premium descriptor for food, often leading consumers to accept higher prices. This financial allure can encourage fraudsters to mislabel or substitute game meat with cheaper domestic pork. This study aimed to use a non-targeted <sup>1</sup>H NMR spectroscopy and/or MALDI-ToF–MS analysis, coupled with multivariate statistics, to distinguish between wild boar (<i>Sus scrofa</i>) and pork (<i>Sus scrofa domesticus</i>) meat. To accomplish this, we collected meat of wild boar, pork, and their processed versions for the analyses of <sup>1</sup>H NMR-based metabolomics (polar and apolar phases; 72 samples) and MALDI-ToF–MS-based proteomics (122 samples). Multivariate data analyses (PCA and OPLS-DA) revealed that pork meat showed higher levels of triglycerides and higher concentrations of certain metabolites such as carnosine, creatine and lactate. Conversely, wild boar meat contained higher levels of n-3 fatty acids (FA), polyunsaturated FA and exhibited higher concentrations of specific amino acids like glutamine and alanine. Additionally, we identified fumaric acid and malic acid as distinctive markers for wild boar meat. Our MALDI-ToF–MS analyses also enabled us to differentiate between wild boar and pork meat. However, when it comes to processed meat, the distinctions became blurred, making it impossible to clearly separate wild boar and pork processed meat. Furthermore, data fusion of <sup>1</sup>H NMR and MALDI-ToF–MS was applied to provide more accurate knowledge about the differentiation of wild boar and pork meat.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Differentiation of pork (Sus scrofa domesticus) and wild boar (Sus scrofa) meat using 1H NMR spectroscopy and MALDI-ToF mass spectrometry

  • 期刊论文

  • 发表日期：

    2025年2月13日

查看全文
  • Liane Wagner,
  • Martin Kaufmann,
  • Frank Lange,
  • André Dallmann,
  • Meike Bergmann

摘要

The term ‘wild’ is a premium descriptor for food, often leading consumers to accept higher prices. This financial allure can encourage fraudsters to mislabel or substitute game meat with cheaper domestic pork. This study aimed to use a non-targeted 1H NMR spectroscopy and/or MALDI-ToF–MS analysis, coupled with multivariate statistics, to distinguish between wild boar (Sus scrofa) and pork (Sus scrofa domesticus) meat. To accomplish this, we collected meat of wild boar, pork, and their processed versions for the analyses of 1H NMR-based metabolomics (polar and apolar phases; 72 samples) and MALDI-ToF–MS-based proteomics (122 samples). Multivariate data analyses (PCA and OPLS-DA) revealed that pork meat showed higher levels of triglycerides and higher concentrations of certain metabolites such as carnosine, creatine and lactate. Conversely, wild boar meat contained higher levels of n-3 fatty acids (FA), polyunsaturated FA and exhibited higher concentrations of specific amino acids like glutamine and alanine. Additionally, we identified fumaric acid and malic acid as distinctive markers for wild boar meat. Our MALDI-ToF–MS analyses also enabled us to differentiate between wild boar and pork meat. However, when it comes to processed meat, the distinctions became blurred, making it impossible to clearly separate wild boar and pork processed meat. Furthermore, data fusion of 1H NMR and MALDI-ToF–MS was applied to provide more accurate knowledge about the differentiation of wild boar and pork meat.