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<p>Hydrogen sulfide (H<sub>2</sub>S) is a crucial gaseous signaling molecule implicated in a wide range of physiological and pathological processes. Accordingly, a fluorescent probe (<b>INS</b>) has been designed and synthesized for selective detection of H<sub>2</sub>S in living cells. Notably, H<sub>2</sub>S triggers a specific nucleophilic reaction with <b>INS</b>, distinguishing it from other potential interfering analytes and conferring exceptional selectivity. The probe exhibits a pronounced fluorescence enhancement upon reaction with H<sub>2</sub>S. Owing to its remarkable properties—including full water solubility, a low detection limit of 30.1&#xa0;nM, excellent specificity, a large Stokes shift of 130&#xa0;nm, a strong linear fluorescence response, and stable emission over a broad pH range (pH 5–12)—<b>INS</b> is ideally suited for the detection of H<sub>2</sub>S in complex biological samples. Cellular imaging experiments further demonstrate that <b>INS</b> possesses high-contrast imaging capabilities and excellent membrane permeability, enabling effective visualization of intracellular H<sub>2</sub>S. Moreover, <b>INS</b> displays organelle-targeting properties, with the ability to localize selectively to both lysosomes and mitochondria. Taken together, these outstanding features position <b>INS</b> as a promising and versatile fluorescent probe for sensitive and selective detection of H<sub>2</sub>S in living cells.</p> Graphical Abstract <p></p>

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Dual-organelle localizing fluorescent probe for selective hydrogen sulfide imaging in mitochondria and lysosomes

  • 期刊论文

  • 发表日期：

    2025年9月25日

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  • Wen-Yu Lu,
  • Ming-Yang Zhang,
  • Xi-Xi Wu,
  • Xing-Yuan Bing,
  • Hui-Jing Li,
  • Yan-Chao Wu

摘要

Hydrogen sulfide (H2S) is a crucial gaseous signaling molecule implicated in a wide range of physiological and pathological processes. Accordingly, a fluorescent probe (INS) has been designed and synthesized for selective detection of H2S in living cells. Notably, H2S triggers a specific nucleophilic reaction with INS, distinguishing it from other potential interfering analytes and conferring exceptional selectivity. The probe exhibits a pronounced fluorescence enhancement upon reaction with H2S. Owing to its remarkable properties—including full water solubility, a low detection limit of 30.1 nM, excellent specificity, a large Stokes shift of 130 nm, a strong linear fluorescence response, and stable emission over a broad pH range (pH 5–12)—INS is ideally suited for the detection of H2S in complex biological samples. Cellular imaging experiments further demonstrate that INS possesses high-contrast imaging capabilities and excellent membrane permeability, enabling effective visualization of intracellular H2S. Moreover, INS displays organelle-targeting properties, with the ability to localize selectively to both lysosomes and mitochondria. Taken together, these outstanding features position INS as a promising and versatile fluorescent probe for sensitive and selective detection of H2S in living cells.

Graphical Abstract