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<p>This work presents the development of a highly sensitive, selective, and efficient aptamer-based fluorescent sensor for detecting cortisol in human urine. Carbon quantum dots-nucleic acid aptamer (CQDs-Apt) synthesized with excellent photoluminescent properties and stability, were selected as the fluorescent probe. In the presence of MoS<sub>2</sub>-NSs, CQDs-Apt adsorbed onto the surface of MoS<sub>2</sub>-NSs via electrostatic and π-π interactions, leading to strong and rapid fluorescence quenching due to static quenching mechanism between them. The CQDs-Apt/MoS<sub>2</sub>-NSs complex can be employed as a “turn-on” fluorescent sensor for cortisol. Upon the addition of cortisol to the CQDs-Apt/MoS<sub>2</sub>-NSs sensor, the aptamer specifically binds to cortisol, thereby weakening the interaction between CQDs-Apt and MoS<sub>2</sub>-NSs. This results in the desorption of CDs-Apt from the surface of MoS<sub>2</sub>-NSs and the recovery of the fluorescence signal. Under optimized conditions, the CQDs-Apt/MoS<sub>2</sub>-NSs sensor exhibits a linear response to cortisol concentration (1–500&#xa0;ng/mL) with a detection limit of 0.3&#xa0;ng/mL. Furthermore, the sensor demonstrated excellent stability, high accuracy (92.0% to 97.7%), and superior precision (RSD ≤ 3.5%). This sensor has achieved a sensitive, rapid and efficient response to cortisol, and been&#xa0;successfully utilized for the detection of cortisol in human urine, demonstrating its potential for clinical cortisol detection.</p>

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Carbon quantum dot-aptamer/MoS2 nanosheet fluorescent sensor for ultrasensitive, noninvasive cortisol detection

  • 期刊论文

  • 发表日期：

    2025年9月9日

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  • Rong Chen,
  • Mingyu Wang,
  • Hailiang Nie,
  • Jinku Zhang,
  • Hongyuan Yan

摘要

This work presents the development of a highly sensitive, selective, and efficient aptamer-based fluorescent sensor for detecting cortisol in human urine. Carbon quantum dots-nucleic acid aptamer (CQDs-Apt) synthesized with excellent photoluminescent properties and stability, were selected as the fluorescent probe. In the presence of MoS2-NSs, CQDs-Apt adsorbed onto the surface of MoS2-NSs via electrostatic and π-π interactions, leading to strong and rapid fluorescence quenching due to static quenching mechanism between them. The CQDs-Apt/MoS2-NSs complex can be employed as a “turn-on” fluorescent sensor for cortisol. Upon the addition of cortisol to the CQDs-Apt/MoS2-NSs sensor, the aptamer specifically binds to cortisol, thereby weakening the interaction between CQDs-Apt and MoS2-NSs. This results in the desorption of CDs-Apt from the surface of MoS2-NSs and the recovery of the fluorescence signal. Under optimized conditions, the CQDs-Apt/MoS2-NSs sensor exhibits a linear response to cortisol concentration (1–500 ng/mL) with a detection limit of 0.3 ng/mL. Furthermore, the sensor demonstrated excellent stability, high accuracy (92.0% to 97.7%), and superior precision (RSD ≤ 3.5%). This sensor has achieved a sensitive, rapid and efficient response to cortisol, and been successfully utilized for the detection of cortisol in human urine, demonstrating its potential for clinical cortisol detection.