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<p>Tetracycline (TC) is a broad-spectrum antibiotic widely used in animal husbandry that may contaminate foodstuffs. In this study, a highly sensitive immunochromatographic assay (ICA) for TC was developed and applied to test meat samples. The assay utilized an indirect competitive format, with conjugates of anti-species antibodies and gold nanoparticles (GNPs) used to label specific immune complexes. The intrinsic peroxidase-like activity of GNPs was leveraged to enhance assay sensitivity. GNPs catalyzed substrate oxidation, resulting in a colored product that increased the brightness of the analytical zones on the test strips. An optimal substrate solution based on 3,3′,5,5′-tetramethylbenzidine was selected. The limit of TC detection reached&#xa0;was 0.03&#xa0;ng/mL. The assay duration was 18&#xa0;min, including the catalytic reaction. Two meat sample preparation procedures were compared. The developed ICA demonstrated TC recoveries in beef ranging from 92 to 113%. The simple, rapid, and sensitive TC detection, combined with the use of GNP labels well-suited to manufacturing technologies, makes the developed ICA an efficient tool for mass screening tests of food products.</p> Graphical abstract <p></p>

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Enhanced immunochromatographic assay of tetracycline using intrinsic peroxidase-like activity of gold nanoparticles

  • 期刊论文

  • 发表日期：

    2025年7月27日

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  • Elena A. Zvereva,
  • Olga D. Hendrickson,
  • Boris B. Dzantiev,
  • Anatoly V. Zherdev

摘要

Tetracycline (TC) is a broad-spectrum antibiotic widely used in animal husbandry that may contaminate foodstuffs. In this study, a highly sensitive immunochromatographic assay (ICA) for TC was developed and applied to test meat samples. The assay utilized an indirect competitive format, with conjugates of anti-species antibodies and gold nanoparticles (GNPs) used to label specific immune complexes. The intrinsic peroxidase-like activity of GNPs was leveraged to enhance assay sensitivity. GNPs catalyzed substrate oxidation, resulting in a colored product that increased the brightness of the analytical zones on the test strips. An optimal substrate solution based on 3,3′,5,5′-tetramethylbenzidine was selected. The limit of TC detection reached was 0.03 ng/mL. The assay duration was 18 min, including the catalytic reaction. Two meat sample preparation procedures were compared. The developed ICA demonstrated TC recoveries in beef ranging from 92 to 113%. The simple, rapid, and sensitive TC detection, combined with the use of GNP labels well-suited to manufacturing technologies, makes the developed ICA an efficient tool for mass screening tests of food products.

Graphical abstract