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<p>A mail-in assessment of vitamin D status will make it possible for the subjects to early detect any vitamin D insufficiency/deficiency at their homes, thus leading to a better management of their health. In this study, a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI–MS/MS) method was developed and validated for quantifying 25-hydroxyvitamin D<sub>3</sub> [25(OH)D<sub>3</sub>], which is the established marker of the vitamin D status, in the finger-prick blood plasma sample prepared by the DEMECAL<sup>®</sup> device. By using the DEMECAL<sup>®</sup> device, the subjects can collect their finger-prick blood at their homes and immediately separate the plasma from the blood by themselves. The DEMECAL<sup>®</sup>-based procedure had advantages over the venipuncture- and dried blood spot-based procedures because it is less invasive and a medical professional-free blood collection, and no need to be concerned with the heterogeneity of the analyte distribution in the sample. The developed LC/ESI–MS/MS method enabled the precise and accurate quantification of 25(OH)D<sub>3</sub> and provided the lower limit of quantification of 0.10&#xa0;ng/mL in the diluted plasma sample due to the derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione. 25(OH)D<sub>3</sub> was stable in the plasma sample prepared by the DEMECAL<sup>®</sup> device at room temperature (23–27&#xa0;°C) for 7&#xa0;days (simulated condition in the mail) and at 4&#xa0;°C for 30&#xa0;days (simulated condition during storage at a laboratory). The measured 25(OH)D<sub>3</sub> concentrations by the DEMECAL<sup>®</sup>-based procedure well agreed with those by the conventional venipuncture-based procedure. These results demonstrated that the derivatization-LC/ESI–MS/MS combined with the DEMECAL<sup>®</sup>-based sample preparation is a promising procedure for the mail-in assessment of the vitamin D status.</p> Graphical Abstract <p></p>

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An LC/MS/MS method for quantifying 25-hydroxyvitamin D3 in finger-prick plasma sample prepared by DEMECAL® micro plasma separation device: toward mail-in assessment of vitamin D status

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月10日

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  • Kodai Maeda,
  • Kyoka Sakamoto,
  • Eiko Ito,
  • Shinya Sugimoto,
  • Shingo Tajima,
  • Takahisa Sasahara,
  • Shunji Kawamura,
  • Kazuo Yaegashi,
  • Tatsuya Higashi

摘要

A mail-in assessment of vitamin D status will make it possible for the subjects to early detect any vitamin D insufficiency/deficiency at their homes, thus leading to a better management of their health. In this study, a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI–MS/MS) method was developed and validated for quantifying 25-hydroxyvitamin D3 [25(OH)D3], which is the established marker of the vitamin D status, in the finger-prick blood plasma sample prepared by the DEMECAL® device. By using the DEMECAL® device, the subjects can collect their finger-prick blood at their homes and immediately separate the plasma from the blood by themselves. The DEMECAL®-based procedure had advantages over the venipuncture- and dried blood spot-based procedures because it is less invasive and a medical professional-free blood collection, and no need to be concerned with the heterogeneity of the analyte distribution in the sample. The developed LC/ESI–MS/MS method enabled the precise and accurate quantification of 25(OH)D3 and provided the lower limit of quantification of 0.10 ng/mL in the diluted plasma sample due to the derivatization with 4-(4-dimethylaminophenyl)-1,2,4-triazoline-3,5-dione. 25(OH)D3 was stable in the plasma sample prepared by the DEMECAL® device at room temperature (23–27 °C) for 7 days (simulated condition in the mail) and at 4 °C for 30 days (simulated condition during storage at a laboratory). The measured 25(OH)D3 concentrations by the DEMECAL®-based procedure well agreed with those by the conventional venipuncture-based procedure. These results demonstrated that the derivatization-LC/ESI–MS/MS combined with the DEMECAL®-based sample preparation is a promising procedure for the mail-in assessment of the vitamin D status.

Graphical Abstract