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<p>Real-time monitoring of sweat lactate provides valuable physiological insights for assessing exercise outcomes and athletic performance. Conventional lactate detection methods, while sensitive, often lack portability and real-time capability for use in wearable or in-body applications. To address these limitations, electrochemical biosensing has emerged as a leading approach, enabling non-invasive and real-time analysis. Wearable devices which integrate lactate-specific enzymes with electrochemical transducers might provide efficient solutions for continuous monitoring. In this study, a wearable lactate biosensor was developed using custom screen-printed electrodes modified with a bio-hybrid probe comprising Prussian blue, carbon black, and lactate oxidase. All the key experimental parameters were optimized, and a detection limit of 60&#xa0;µM and a linearity up to 20&#xa0;mM were obtained. A filter paper-based strip was incorporated to enhance sweat collection and serve as the real sample collector by exploiting its porosity: this configuration allowed a satisfactory repeatability of 6%. The system was validated using real sweat samples, highlighting a quantitative correlation (94–103%) with LC–MS/MS measurements. The biosensor was integrated onto a 3D-printed thermoplastic polyurethane (TPU) armband, designed for a customizable and comfortable fit, ensuring effective sweat collection and transport. This low-cost, wearable system represents a significant step forward in non-invasive, continuous, and personalized health monitoring, providing a practical tool for tracking physiological parameters in real-time.</p>

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On-body electrochemical measurement of sweat lactate with the use of paper-based fluidics and 3D-printed flexible wearable biosensor

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月9日

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  • Gabriella Iula,
  • Antonella Miglione,
  • Panagiota M. Kalligosfyri,
  • Michele Spinelli,
  • Angela Amoresano,
  • Concetta Di Natale,
  • Ibrahim A. Darwish,
  • Stefano Cinti

摘要

Real-time monitoring of sweat lactate provides valuable physiological insights for assessing exercise outcomes and athletic performance. Conventional lactate detection methods, while sensitive, often lack portability and real-time capability for use in wearable or in-body applications. To address these limitations, electrochemical biosensing has emerged as a leading approach, enabling non-invasive and real-time analysis. Wearable devices which integrate lactate-specific enzymes with electrochemical transducers might provide efficient solutions for continuous monitoring. In this study, a wearable lactate biosensor was developed using custom screen-printed electrodes modified with a bio-hybrid probe comprising Prussian blue, carbon black, and lactate oxidase. All the key experimental parameters were optimized, and a detection limit of 60 µM and a linearity up to 20 mM were obtained. A filter paper-based strip was incorporated to enhance sweat collection and serve as the real sample collector by exploiting its porosity: this configuration allowed a satisfactory repeatability of 6%. The system was validated using real sweat samples, highlighting a quantitative correlation (94–103%) with LC–MS/MS measurements. The biosensor was integrated onto a 3D-printed thermoplastic polyurethane (TPU) armband, designed for a customizable and comfortable fit, ensuring effective sweat collection and transport. This low-cost, wearable system represents a significant step forward in non-invasive, continuous, and personalized health monitoring, providing a practical tool for tracking physiological parameters in real-time.