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<p>As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the&#xa0;biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG<sub>6</sub>-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC<sup>2</sup>-MS/MS) assay was firstly developed and validated for the&#xa0;determination of M-PEG<sub>6</sub>-PA polymers in a&#xa0;biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of <i>m</i>/<i>z</i> 367.2→118.9 was used to quantify M-PEG<sub>6</sub>-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC<sup>2</sup>-MS/MS assay for quantification of M-PEG<sub>6</sub>-PA in a&#xa0;biological matrix is 0.05 to 30 μg/mL (<i>R</i>≥0.995). Intra-day and inter-day precisions for the&#xa0;determination of M-PEG<sub>6</sub>-PA by this analytical assay were &lt;6.99%. The absolute recoveries and matrix effects of M-PEG<sub>6</sub>-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC<sup>2</sup>-MS/MS assay was successfully applied to quantify the concentrations of M-PEG<sub>6</sub>-PA polymers in rat plasma samples.</p> Graphical Abstract <p></p>

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Development and validation of a high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry assay for quantification of methoxy-polyethylene glycol propionic acid polymers

  • 期刊论文

  • 发表日期：

    2025年3月26日

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  • Chunpeng Feng,
  • Jiye Tian,
  • Qisheng Fang,
  • Yajie Cheng,
  • Yue Deng,
  • JiaRui Zhang,
  • Shuang Feng,
  • Qingbin Wang,
  • Hecheng Wang,
  • Xuan Zhao,
  • Lei Yin

摘要

As a synthetic polymer, methoxy-polyethylene glycol propionic acid (M-PEG-PA) is widely used in the biomedicine field. Unraveling the pharmacokinetic behavior of M-PEG6-PA in vivo is crucial for evaluating the safety and efficiency of M-PEG-PA-related polymers or drug delivery systems. A high-throughput and green ultra-performance convergence chromatography-tandem mass spectrometry (UPC2-MS/MS) assay was firstly developed and validated for the determination of M-PEG6-PA polymers in a biological matrix. The MRM transition (mass to charge ratio, precursor ions→fragment ions) of m/z 367.2→118.9 was used to quantify M-PEG6-PA in this study. The throughput of the assay is high and the total running time for each sample was only 2 min. The linear range of the developed UPC2-MS/MS assay for quantification of M-PEG6-PA in a biological matrix is 0.05 to 30 μg/mL (R≥0.995). Intra-day and inter-day precisions for the determination of M-PEG6-PA by this analytical assay were <6.99%. The absolute recoveries and matrix effects of M-PEG6-PA ranged from 79.50 to 92.47% and 68.72 to 81.73%, respectively. The UPC2-MS/MS assay was successfully applied to quantify the concentrations of M-PEG6-PA polymers in rat plasma samples.

Graphical Abstract