Scholarlink

<p>Vanadyl acetylacetonate, VO(acac)<sub>2</sub>, is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac)<sub>2</sub> system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac)<sub>2</sub> CL was used in the&#xa0;quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100&#xa0;μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5&#xa0;μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples.</p> Graphical Abstract <p></p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Aerobic oxidation catalyst vanadyl acetylacetonate boosts luminol chemiluminescence for sensitive detection of alkaline phosphatase and ascorbic acid

  • 期刊论文

  • 发表日期：

    2025年3月17日

查看全文
  • Wei Zhang,
  • Yequan Chen,
  • Kaixiang Ji,
  • Zhiyong Dong,
  • Abubakar Abdussalam,
  • Yuriy T. Zholudov,
  • Wenyue Gao,
  • Yu Tian,
  • Guobao Xu

摘要

Vanadyl acetylacetonate, VO(acac)2, is a well-known environmentally friendly catalyst for aerobic oxidations. It has been exploited as an efficient co-reactant of luminol chemiluminescence (CL) in aqueous media for the first time. The luminol-VO(acac)2 system generates an intense CL and ascorbic acid can quench its CL remarkably. Based on the strong quenching effect of ascorbic acid, the luminol-VO(acac)2 CL was used in the quantification of clinically important ascorbic acid and alkaline phosphatase (ALP) by enzymatic conversion of L-ascorbic acid 2-phosphate into ascorbic acid. The linear ranges for detecting ascorbic acid and alkaline phosphatase are 1 to 100 μM and 0.005 to 200 U/L, respectively. The limits of detection of ascorbic acid and alkaline phosphatase are 0.5 μM and 0.005 U/L, respectively. The proposed CL method has shown high selectivity for the ALP among the tested biomolecules and metal ions. It also shows good recoveries in human serum samples.

Graphical Abstract