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<p>Breast cancer (BC) is a leading cause of cancer-related mortality, with estrogen receptor (ER)-negative subtypes, especially triple-negative BC, comprising one-fifth of global cases. Natural inhibitors, particularly those from cruciferous vegetables like arugula (<i>Eruca sativa</i>), which are rich in bioactive isothiocyanates (ITCs), show potent anticancer effects and cytoprotection when combined with chemotherapy. Sulforaphane (SFN) and its analogue erucin modulate oxidative stress, detoxification, and epigenetic pathways. This study computationally assessed their anti-cancer potential in ER-negative BC using transcriptomic analysis, molecular docking, and ADMET profiling. Microarray data (GSE28813) from SFN-treated ER-negative MCF10A cells were analyzed via GEO2R and GEOExplorer to identify highly upregulated differentially expressed genes (DEGs). Key DEGs included AKR1B10 (logFC = 7.26), AKR1C1 (logFC = 5.10), AKR1C3 (logFC = 4.42), NMRAL1P1 (logFC = 6.42), and HKDC1 (logFC = 6.13). Elevated AKR1B10 is strongly linked to early BC malignancies, positioning it as a diagnostic and therapeutic target. Molecular docking showed SFN’s superior binding affinity to AKR1B10 compared to erucin, with strong interactions at catalytic site residues via hydrogen and hydrophobic bonds. ADMET profiling confirmed SFN’s high intestinal absorption and blood–brain barrier non-permeability. Thus, integrating SFN as a natural AKR1B10 inhibitor into ER-negative BC treatment regimens may enhance early malignancy management and support its development as a nutraceutical adjunct.</p>

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Arugula-derived isothiocyanates as novel agents for a potential regulator of AKR1B10 protein in breast cancer: an integrated transcriptomic and molecular docking approach

  • 期刊论文

  • 发表日期：

    2025年12月27日

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  • Nosyba A. Ibrahim,
  • Huda A. Sherfi,
  • Faizul Azam,
  • Eyad Al Shammari,
  • Mirza Sarwar Baig,
  • Syed Amir Ashraf

摘要

Breast cancer (BC) is a leading cause of cancer-related mortality, with estrogen receptor (ER)-negative subtypes, especially triple-negative BC, comprising one-fifth of global cases. Natural inhibitors, particularly those from cruciferous vegetables like arugula (Eruca sativa), which are rich in bioactive isothiocyanates (ITCs), show potent anticancer effects and cytoprotection when combined with chemotherapy. Sulforaphane (SFN) and its analogue erucin modulate oxidative stress, detoxification, and epigenetic pathways. This study computationally assessed their anti-cancer potential in ER-negative BC using transcriptomic analysis, molecular docking, and ADMET profiling. Microarray data (GSE28813) from SFN-treated ER-negative MCF10A cells were analyzed via GEO2R and GEOExplorer to identify highly upregulated differentially expressed genes (DEGs). Key DEGs included AKR1B10 (logFC = 7.26), AKR1C1 (logFC = 5.10), AKR1C3 (logFC = 4.42), NMRAL1P1 (logFC = 6.42), and HKDC1 (logFC = 6.13). Elevated AKR1B10 is strongly linked to early BC malignancies, positioning it as a diagnostic and therapeutic target. Molecular docking showed SFN’s superior binding affinity to AKR1B10 compared to erucin, with strong interactions at catalytic site residues via hydrogen and hydrophobic bonds. ADMET profiling confirmed SFN’s high intestinal absorption and blood–brain barrier non-permeability. Thus, integrating SFN as a natural AKR1B10 inhibitor into ER-negative BC treatment regimens may enhance early malignancy management and support its development as a nutraceutical adjunct.