Scholarlink

<p>Bladder cancer (BLCA) is one of the most frequent malignant tumors worldwide, with markedly poor prognosis when distant metastasis occurs. Long noncoding RNA (lncRNA) TRPM2-AS has been reported to play an oncogenic role in several cancers. Although TRPM2-AS was previously revealed to suppress BLCA cell growth, its role in BLCA cell metastasis remains largely unknown.&#xa0;In our study,&#xa0;TRPM2-AS, miR-195-5p, and COP1 relative expression in BLCA cells was estimated by RT-qPCR. Through MTT, EdU, colony formation, wound-healing, Transwell, and western blotting assays, the biological effects of TRPM2-AS and COP1 on BLCA cell proliferation, migration, invasion, and EMT were evaluated. Target association between miR-195-5p and TRPM2-AS (or COP1) was confirmed by RNA pull-down, RIP, and luciferase activity assays. The nucleocytoplasmic localization of TRPM2-AS in BLCA cells was measured by FISH assay.&#xa0;Xenograft mouse models of BLCA were used to validate the role of TRPM2-AS on tumor growth and EMT <i>in vivo</i>.&#xa0;Our results showed that&#xa0;TRPM2-AS and COP1 expression was increased, while miR-195-5p expression was decreased in BLCA cells and tissues. TRPM2-AS silencing repressed BLCA cell growth, migration, invasion, and EMT. TRPM2-AS acted as a competing endogenous RNA (ceRNA) to spongemiR-195-5p, thereby positively regulating COP1 expression and activating the PI3K/AKT signaling. Overexpressing COP1 antagonized the influence of TRPM2-AS knockdown on BLCA cell growth, migration, invasion, and EMT. Additionally, TRPM2-AS knockdown inhibited tumor growth, EMT, and the PI3K/AKT signaling, attenuated COP1 expression, and enhanced miR-195-5p expression in xenograftmouse models.&#xa0;Collectively,&#xa0;TRPM2-AS functions as an oncogene in BLCA development via the miR-195-5p/COP1 axis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

LncRNA TRPM2-AS promotes cell proliferation, migration, and invasion by regulating the miR-195-5p/COP1 axis in bladder cancer

  • 期刊论文

  • 发表日期：

    2025年6月23日

查看全文
  • Changyuan Dai,
  • Qingwen Li,
  • Lili Wang,
  • Jiajun Zhang,
  • Shuai Yang,
  • Xiaole Zhang

摘要

Bladder cancer (BLCA) is one of the most frequent malignant tumors worldwide, with markedly poor prognosis when distant metastasis occurs. Long noncoding RNA (lncRNA) TRPM2-AS has been reported to play an oncogenic role in several cancers. Although TRPM2-AS was previously revealed to suppress BLCA cell growth, its role in BLCA cell metastasis remains largely unknown. In our study, TRPM2-AS, miR-195-5p, and COP1 relative expression in BLCA cells was estimated by RT-qPCR. Through MTT, EdU, colony formation, wound-healing, Transwell, and western blotting assays, the biological effects of TRPM2-AS and COP1 on BLCA cell proliferation, migration, invasion, and EMT were evaluated. Target association between miR-195-5p and TRPM2-AS (or COP1) was confirmed by RNA pull-down, RIP, and luciferase activity assays. The nucleocytoplasmic localization of TRPM2-AS in BLCA cells was measured by FISH assay. Xenograft mouse models of BLCA were used to validate the role of TRPM2-AS on tumor growth and EMT in vivo. Our results showed that TRPM2-AS and COP1 expression was increased, while miR-195-5p expression was decreased in BLCA cells and tissues. TRPM2-AS silencing repressed BLCA cell growth, migration, invasion, and EMT. TRPM2-AS acted as a competing endogenous RNA (ceRNA) to spongemiR-195-5p, thereby positively regulating COP1 expression and activating the PI3K/AKT signaling. Overexpressing COP1 antagonized the influence of TRPM2-AS knockdown on BLCA cell growth, migration, invasion, and EMT. Additionally, TRPM2-AS knockdown inhibited tumor growth, EMT, and the PI3K/AKT signaling, attenuated COP1 expression, and enhanced miR-195-5p expression in xenograftmouse models. Collectively, TRPM2-AS functions as an oncogene in BLCA development via the miR-195-5p/COP1 axis.