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<p>Foodborne pathogens such as <i>Staphylococcus aureus</i> and <i>Listeria monocytogenes</i> pose serious public health risks due to their antibiotic resistance and biofilm-forming abilities. This study aimed to characterise the antimicrobial activity of the exopolysaccharide (EPS) produced by <i>Lentilactobacillus buchneri</i> KU200793 against <i>S. aureus</i> and <i>L. monocytogenes</i>, as well as its mechanism of action. Purified EPS from <i>L. buchneri</i> KU200793 appeared as a white, water-soluble powder with a yield of 575&#xa0;mg/L in de Man, Rogosa, and Sharp broth supplemented with 5% sucrose. Ultraviolet–visible spectroscopy confirmed its purity, while Fourier transform infrared analysis identified characteristic polysaccharide functional groups, including hydroxyl, carboxyl, and glycosidic bonds. Morphological examination revealed irregular porous structures, and monosaccharide analysis indicated that EPS KU200793 is a heteropolysaccharide composed of mannose, ribose, rhamnose, glucosamine, glucuronic acid, glucose, galactose, and fucose. The EPS exhibited strong antibacterial activity, with a minimum inhibitory concentration (MIC) of 2&#xa0;mg/mL against <i>S. aureus</i> KFRI 00188 and <i>L. monocytogenes</i> ATCC 3596, a minimum bactericidal concentration (MBC) of 4&#xa0;mg/mL, and a 50% lethal concentration of 8&#xa0;mg/mL. The kill kinetics and fluorescence assays confirmed the time- and concentration-dependent bactericidal effects associated with membrane disruption. Scanning electron microscopy (SEM) revealed significant morphological damage, including cell rupture and shrinkage. EPS KU200793 inhibited biofilm formation of <i>S. aureus</i> (up to 61.36%) and <i>L. monocytogenes</i> (up to 74.57%) in a dose-dependent manner. Overall, <i>L. buchneri</i>-derived EPS KU200793 exhibited potent antimicrobial and antibiofilm activities, suggesting its potential as a natural biopolymer for controlling foodborne pathogens in food preservation and safety applications.</p>

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Characterisation and mechanism of action of the antimicrobial activity of hetero-exopolysaccharide from Lentilactobacillus buchneri KU200793 against Staphylococcus aureus and Listeria monocytogenes

  • 期刊论文

  • 发表日期：

    2026年3月30日

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  • Manoj Kumar Yadav,
  • Ji Hoon Song,
  • Robie Vasquez,
  • Jae Seung Lee,
  • Young-Seo Park,
  • Hyun-Dong Paik,
  • In Ho Kim,
  • Dae-Kyung Kang

摘要

Foodborne pathogens such as Staphylococcus aureus and Listeria monocytogenes pose serious public health risks due to their antibiotic resistance and biofilm-forming abilities. This study aimed to characterise the antimicrobial activity of the exopolysaccharide (EPS) produced by Lentilactobacillus buchneri KU200793 against S. aureus and L. monocytogenes, as well as its mechanism of action. Purified EPS from L. buchneri KU200793 appeared as a white, water-soluble powder with a yield of 575 mg/L in de Man, Rogosa, and Sharp broth supplemented with 5% sucrose. Ultraviolet–visible spectroscopy confirmed its purity, while Fourier transform infrared analysis identified characteristic polysaccharide functional groups, including hydroxyl, carboxyl, and glycosidic bonds. Morphological examination revealed irregular porous structures, and monosaccharide analysis indicated that EPS KU200793 is a heteropolysaccharide composed of mannose, ribose, rhamnose, glucosamine, glucuronic acid, glucose, galactose, and fucose. The EPS exhibited strong antibacterial activity, with a minimum inhibitory concentration (MIC) of 2 mg/mL against S. aureus KFRI 00188 and L. monocytogenes ATCC 3596, a minimum bactericidal concentration (MBC) of 4 mg/mL, and a 50% lethal concentration of 8 mg/mL. The kill kinetics and fluorescence assays confirmed the time- and concentration-dependent bactericidal effects associated with membrane disruption. Scanning electron microscopy (SEM) revealed significant morphological damage, including cell rupture and shrinkage. EPS KU200793 inhibited biofilm formation of S. aureus (up to 61.36%) and L. monocytogenes (up to 74.57%) in a dose-dependent manner. Overall, L. buchneri-derived EPS KU200793 exhibited potent antimicrobial and antibiofilm activities, suggesting its potential as a natural biopolymer for controlling foodborne pathogens in food preservation and safety applications.